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 <= b>页眉
= 249;<= b>日期/<= b>时间›
‹页脚›
‹#›=
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第= 23章   
分子生物学常用= 216;术
与人类基因组计= 010;
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一、分子杂交和= 印渍技术的原理
（一）分子杂交
       DNA= 双链分子加热变性解= ;链后，如控制好条件，缓慢降温，两条= ;链之间可以根据碱基&#= 20114;补的方式再重新形成双链= ;。（复性）
=         如把不同的DNA单链分子放在同一溶= ;液中，或把DNA与RNA放在一起，只要在DNA或RNA的单链分子之间存在= ;着一定程度的碱基配= 对关系，就可在不同= ;的分子间形成杂化双&#= 38142;，= 这就叫核酸分子杂交<= span style=3D'font-family:SimSun;mso-fareast-font-family:SimSun;font-size:133%'= >。
第一节 分子杂交ߎ= 2;印渍（迹）技术
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用๨= 4;
•<= /span>研究DNA分子中某一种基因的= ;位置，两种核酸分子间= ;的相似性即同源性; =
•<= /span>也可用于检测某些专= ;一序列在待= 检样品中的存在与否= ;等。
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（二）印渍技术
1<= /span>、Edwen = Southern  1975年首&= #20808;提出
        琼脂糖分离的DNA片段 → 变性成= 为单链 → 将一张= NC= 膜放在胶上，上面放= ;吸水纸巾 → 利用毛= 细作用使胶中的DNA片段转移到NC= 膜上，使之成为固相= ;化分子。
       载有DNA单链分子的NC= 膜就可以在杂交液与另一种DNA或RNA分子（探针）进行杂= ;交，具有互补序列的= ;RNA或DNA结合到存在于NC= 膜的DNA分子上，经过检测分= ;析就可以显现出杂交分子的区= ;带。
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2<= /span>、定义
      是将存在于凝胶中的= ;生物大分子转移（印渍）于固化介质上并= ;加以检测分析的技术&#= 12290;
3<= /span>、应用
      广泛用于DNA、RNA、蛋白质的检测
4<= /span>、发展
      电转移印渍技术、真= ;空吸引转移印渍等
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（三）探针技术
1<= /span>、探针（probe)= ：一小段已知序列的= ;用同位素、生物素或荧光染= ;料标记它的末端或全&#= 38142;= 的多聚核苷酸链。
2<= /span>、探针技术：将探针= ;与固定在NC= 膜 上的DNA或RNA进行结合反应，探针= ;的序列如果与NC= 膜上的核酸序列相互= ;补，就可以结合到膜&#= 19978;的相应区带，经放射自= ;显影或其他检测手段&#= 23601;可以叛断膜 上是否= 有同源的核酸分子存= 2312;。
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二、印渍৙= 6;术的类别及应用
（一）DNA印渍技术(South= ern Blot)
（二）RNA印渍技术(North= ern Blot)
（三）蛋白质= 的印渍技术(Weste= rn Blot)
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= Southern印迹杂交法
M<= /span>
1<= /span>
2<= /span>
=
10×SSC<= span lang=3DEN-US style=3D'font-family:"Times New Roman";mso-ascii-font-family:= "Times New Roman"; mso-fareast-font-family:KaiTi_GB2312;font-size:83%;mso-fareast-language:ZH= -CN'>   = 转移缓冲液
Whatman滤纸
凝胶
Whatman滤纸
纸巾
玻璃板
重物
支持物
500g
1<= /span>
2<= /span>
与探针= 516;源杂交的= 基因DNA片段<= /span>
=
基因组DNA
=
DNA酶切片段
内切酶
NC或尼龙膜
NC膜或尼龙膜
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Southert Blot 用途
•<= /span>用于基因组DNA的分析，如在基因组= ;中特异的定位及检测= ;等; =
•可用于分析重组质粒= ;和噬菌体
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（二）RNA印渍技术
原理同= DNA印渍技术
Northern Blot 用途
•<= /span>用于检测某一组织或= ;细胞中已知的特异mRNA的表达水平
•<= /span>比较不同组织和细胞= ;的同一基因的表达情况。
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（三）蛋白质的印渍= ;技术（免疫印渍）
      首先将蛋白质用PAGE按分子大小分开，再将蛋白质转移= ;到NC= 膜上，蛋白质的位置可以保持在胶中= ;的原位上。
与DNA和RNA印渍相似之处：<= /i>
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•<= /span> <= /b>= 蛋白质的转移只有靠= 电转移方可完成
•<= /span> <= /b>= 蛋白质的检测是以抗= 体做探针的
•<= /span>= 反应时用碱性磷酸酶= （ALP= = ）、辣根过氧化酶= 标记（HRP= = ），底物显色来检测= 蛋白区带的信= 号，底物亦可与化学= 发光剂结合以提高敏= 4863;= = 度。
•<= /span>= 或用同位素标记第二= 抗体，反应之后用放= 3556;自= = 显影来检测蛋白区带= 的信号
与DNA和RNA印渍不同之处：<= /i>
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Western Blot应用
•<= /span>用于检测样品中的特= ;异蛋白质的存在
•<= /span>细胞中特异蛋白质的= ;定量分析
•<= /span>蛋白质分子的相互作= ;用研究等。
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第二节     PCR<= span style=3D'font-family:SimSun;mso-fareast-font-family:SimSun;font-size:183%; color:red'>技术
polymerase chain reaction (PCR)，聚合链ঁ= 5;反应
何谓PCR？
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The Nobel Prize in Physiology or Medicine 1993
= 3;
"for contributions to the developments of methods within DNA-based chemistry"
Michael Smith
1944 -
1932 - 2000
La Jolla, CA, USA
3D"Kary 3D"Michael
Kary B. M= ullis
University of British Columbia
Vancouver, Canada
"f= or his invention of the polymerase chain r= eaction = (PCR) method= "
"for his fundam= ental contrib= utions to the establishment of oligonucleotide-base= d, site-di= rected mutagen= esis and its development for protein studies"= ;
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Content-Location: file:///C:/85899A73/23.files/slide0041.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" PowerPoint 演示文稿
B
A
B
B
A
B
A
A
B
A
B
A
B
A
B
A
靶= 4207;列
未扩增&= #30340;DNA=
循环1
变ঝ= 5;
退ଽ= 9;
延߬= 0;
循环2
变ঝ= 5;、退火
延߬= 0;
A
上游引物
B
下游= 341;物
PCR示意&= #22270;
5 '端是人工合= 5104;的引= 物，是特定的，= 3 '端没有固定的终止点=
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R0lGODlhjAAdAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALCEAAQAG ABYAgAAAAP8A/wIZBBJmqJfK4FvTtUhvxVbq7znISFLRCC5KAQA7 ------=_NextPart_01C48438.43A1D1D0 Content-Location: file:///C:/85899A73/23.files/slide0015.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" PowerPoint 演示文稿
25至30= 次循环，
DNA的含量可扩大100万倍
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二、PCR的主要用途
（一）目的基因的克= ;隆
1 = 利用特异性引物以cDNA或基因组DNA为模板获得已知的目的基= ;因片段；
2 = 利用简并引物从cDNA文库或基因组文库中= ;获得具有一定同源性的= ;基因片段；
3 = 利用随机引物从cDNA文库或基因组文库中= ;随机克隆基因。
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（二）基因的体外突= ;变
（三）DNA的微量分析
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第三节   核酸序列分析<= /div>
概     论
1<= /span>、重要性
2<= /span>、发展历史
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一、化学裂解法= (Maxam-Gilber法)
1  原理<= /b>
32P
仅一端带੍= 7;放射性标记的靶DNA
G    (A+G)    (C+T)     (C+T)     A>C
进行5组碱= 基特异性的裂解反应
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32P
ACACT<= /b>GAACGTT= CATGTCGA…= ;…
me
例如，用ఊ= 7;酸二甲酯对G残基= 进行= 部分修饰
32P
ACACT<= /b>GAACGTTCATG= TCGA…= ;…
me
32P
ACACT<= /b>GAACGTTCAT<= /b>GTCGA…= ;…
me
32P
ACACT<= /b>GAACGTTCAT<= /b>GTC= GA<= /span>…= ;…
me
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用哌啶处ச= 2;，使被修饰的G残基= 脱落并= 使糖—磷酸= 主链发生水解
32P
ACACT<= /b>
32P
ACACTG= AAC
32P
ACACTG= AACGTTCAT
32P
ACACTG= AACGTTCATGTC
 通过PAGE分析放射ঝ= 5;标记的DNA片段=
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G
(A+G)
C
(C+T)<= /span>
A>C
 AC<= span lang=3DEN-US style=3D'font-size:75%;mso-fareast-language:ZH-CN'>A<= span lang=3DEN-US style=3D'font-size:75%;mso-fareast-language:ZH-CN'>CT<= span lang=3DEN-US style=3D'font-size:75%;mso-fareast-language:ZH-CN'>G<= span lang=3DEN-US style=3D'font-size:75%;mso-fareast-language:ZH-CN'>A<= span lang=3DEN-US style=3D'font-size:75%;mso-fareast-language:ZH-CN'>A<= span lang=3DEN-US style=3D'font-size:75%;mso-fareast-language:ZH-CN'>C<= span lang=3DEN-US style=3D'font-size:75%;mso-fareast-language:ZH-CN'>G<= span lang=3DEN-US style=3D'font-size:75%;mso-fareast-language:ZH-CN'>T<= span lang=3DEN-US style=3D'font-size:75%;mso-fareast-language:ZH-CN'>TC<= span lang=3DEN-US style=3D'font-size:75%;mso-fareast-language:ZH-CN'>A<= span lang=3DEN-US style=3D'font-size:75%;mso-fareast-language:ZH-CN'>T<= span lang=3DEN-US style=3D'font-size:75%;mso-fareast-language:ZH-CN'>G<= span lang=3DEN-US style=3D'font-size:75%;mso-fareast-language:ZH-CN'>T<= span lang=3DEN-US style=3D'font-size:75%;mso-fareast-language:ZH-CN'>C<= span lang=3DEN-US style=3D'font-size:75%;mso-fareast-language:ZH-CN'>G<= span lang=3DEN-US style=3D'font-size:75%;mso-fareast-language:ZH-CN'>A
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Maxam<= /b>—= ;Gillbe= rt法所用的化学修饰技= ;术
碱基
特异修饰方法
G<= /span>
在pH8.0<= /b>下，用硫酸二甲酯对= ;N<= /span>7进行甲= 基化，使C<= /span>8-C9键对碱= 基裂解具有特异的敏= 4863;性
A+G
在pH2.0<= /b>下，哌啶甲酸可以使= ;嘌呤环的N<= /span>原子质子化，从而导致脱嘌= ;呤，并因此削弱腺嘌&#= 21604;和鸟嘌呤的糖苷键
C+T
胼可打开嘧啶环，后= ;者重新环成五员环后&#= 26131;于除去
C<= /span>
在1.5mol= /LNaCl存在下，只有胞嘧啶= ;可同时胼发生明显可见的反应
A>C=
在90 oC下，用1.2mol= /LNaOH处理可使A<= /span>位点发生剧烈的断裂反应而C<= /span>位点的断裂较微弱
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Maxam-= Gilbert法的最在的优点：
       所测序列来自原DNA分子而不是酶促反应所产生的拷贝= ;；因此，利用该法可对合成的寡核苷酸= ;进行测序，可以分析诸如甲基化等DNA修饰的情况，还可以通过化学保护及= ;修饰干扰实验来研究D N A = 二级结构及蛋白质与= ;DNA的相互作用。
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二、DNA链末端合成终止= 861;
    (Sanger双脱氧链终止法= )
HO   P   O   P   O   P   O   CH2
O<= /span>
O<= /span>
O<= /span>
OH=
OH=
OH=
O<= /span>
A, C, = G  or T
1<= /span>′
3 = ′
OH
α
β
γ
5 = ′
双脱氧核苷三磷酸
脱去
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1<= /span>、原理
   Sanger法使用特异性引物与= ;单链DNA模板相退火
，在DNA聚合酶作用下进行延= ;伸反应，并在双脱 <= /span>
氧核苷= 三磷酸(ddNTP= )作用下进行碱基特异= ;性的链
终止，然后采用聚丙= ;烯酰胺凝胶电泳区分&#= 38271;度相
差一个核苷酸的单链= ;DNA，从而完成的测序反= ;应
方法。=
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DNA自= 动测序技术飞速发展
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第四节    <= /b>人类基因组计划= 982;后基因组研究
基ࢰ= 0;组
•<= /span>在个体水平代表个体= ;所有遗传性状的总和&#= 65307;
•<= /span>在细胞水平，是一个= ;细胞所有染色体的总= 和；
•<= /span>从分子角度认识，它= ;代表了一个物种的所&#= 26377;DNA分子的总和。
人： 23条染色= 体组成，约30= 亿对核苷酸的DNA= 3;
    分子构成，可编码约= ;3-4万个基因
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=
 
=
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AFFFFABRRRQAUUUUAFFFFABRRRQAUUUUAFFFFABRRRQAUUUUAFFFFABRRRQAUUUUAFFFFABRRRQA UUUUAFFFFABRRRQAUUUUAFFFFAH/2Q== ------=_NextPart_01C48438.43A1D1D0 Content-Location: file:///C:/85899A73/23.files/slide0028.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" PowerPoint 演示文稿
一、人类基因组#= 745;划(<= /span>Human <= /b>Genome = Project= )
1986年，美国学者提出了= ;进行HGP研究的设想
1991年，美国众院批准了= ;一个15= 年的人类基因= 组研究计划，总资助= ;额为30= 亿美元
1994年，我国启动了相关= ;研究项目
我国人类基因组南方= ;小组、北方小组
2000年6<= /span>月26= 日，美、英、日、法= ;、德和中国等六国宣布，人类历= ;史上最重要的科研工&#= 31243;= “人类基= 因组”草图绘= 制成功
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（一）人类基因组分= ;析的主要的内容
= 1.遗传图= 分析
= 2.物理图= 分析
= 3.转录图= 分析
= 4.基因组= 序列测定
<= span style=3D'font-size:133%;font-weight:bold'>5.资料的= 储存和利用
= 6.
（二）人类基因组研= ;究在医学上的价值
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（一）后基因组时代= ;研究内容
1.功能基= 因组学
2.蛋白质= 组学
（二）后基因组研究= ;在医学发展中的意义
二、后基因组研= 350;
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第五节   疾病相关基因的= 811;隆与鉴定
（一）功能性克隆
        功能克隆（functi= onal cloning)是指从对一种致病基因的功= ;能的了解出发克隆该致病基因。
         主要用于某些= 生物化学机制已经明= 0830;，基因表达产物较易得= ;到部分纯化的遗传性&#= 30142;病。
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（二）定位克隆
        定位克隆（positi= onal cloning)是指从一种致病基因的染色体定= ;位出发逐步缩小范围&#= 65292;最后克隆该基因。实现= ;从染色体定位到基因&#= 20811;隆是80= 年代医学分子生物学= ;的重要进展之一。
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第六节  转基因、核转移= 982;基因剔除技术
一、转基因技术
二、核转移技术
三、基因剔除技术
四、基因转移和基因= ;剔除在医学中的应用
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 <= b>页眉
= 249;<= b>日期/<= b>时间›
单击此处编辑母版ă= 91;本样式
第二级
第三级
第四级
第五级
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if( !g_supportsPPTHTML ) { =09 sld.style.visibility=3D"visible" return } if( MakeNotesVis() ) return runAnimations =3D _InitAnimations(); =09 if( IsWin("PPTSld") ) parent.SldUpdated(GetSldId()) g_origSz=3DparseInt(SlideObj.style.fontSize) g_origH=3Dsld.style.posHeight g_origW=3Dsld.style.posWidth g_scaleHyperlinks=3D(document.all.tags("AREA").length>0) if( g_scaleHyperlinks ) InitHLinkArray() if( g_scaleInFrame||(IsWin("PPTSld") && parent.IsFullScrMode() ) ) document.body.scroll=3D"no" _RSW() if( IsWin("PPTSld") && parent.IsFullScrMode() ) FullScrInit(); =09 MakeSldVis(); ChkAutoAdv() if( runAnimations ) { if( document.all("NSPlay") ) document.all("NSPlay").autoStart =3D false; if( sld.filters && sld.filters.revealtrans ) setTimeout( "document.body.start()", sld.filters.revealtrans.duration * = 1000 ); else document.body.start(); } } function MakeSldVis()=20 { var fTrans=3Dg_showAnimation && SldHasTrans() if( fTrans )=09 { if( g_bgSound ) { idx=3Dg_bgSound.indexOf(","); pptSound.src=3Dg_bgSound.substr( 0, idx ); pptSound.loop=3D -(parseInt(g_bgSound.substr(idx+1))); } SlideObj.filters.revealtrans.Apply()=09 } SlideObj.style.visibility=3D"visible" if( fTrans ) SlideObj.filters.revealtrans.Play() } function MakeNotesVis()=20 { if( !IsNts() ) return false=20 SlideObj.style.display=3D"none" nObj =3D document.all.item("NotesObj") parent.SetHasNts(0) if( nObj ) {=20 nObj.style.display=3D"" parent.SetHasNts(1) } return 1 } function ChkAutoAdv() { if(SldHasTrans()) SlideObj.onfilterchange=3DAutoAdv else AutoAdv() } function AutoAdv() { if(!IsWin("PPTSld") || !gUseSldTimings )return var sld=3DGetCurSld() if( (sld.mAdvDelay>0) && !parent.IsFramesMode() ) setTimeout("parent.GoToNextSld()",sld.mAdvDelay) } function GetObj(id) { if(g_supportsPPTHTML) return document.all(id); else return document.getElementById(id); } function SldHasTrans() { return SlideObj.style.filter !=3D ""; } function GetSldId() { return sId=3Dlocation.href.substring(location.href.la= stIndexOf('/')+1) } function HideMenu() { if( frames["PPTSld"] && PPTSld.document.all.item("ctx= tmenu") && PPTSld.ctxtmenu.style.display!=3D"none" ) { PPTSld.ctxtmenu.styl= e.display=3D'none'; return true } return false } function IsWin( name ) { return window.name =3D=3D name } function IsNts() { return IsWin("PPTNts") } function IsSldOrNts() { return( IsWin("PPTSld")||IsWin("PPTNts") ) } function SupportsPPTAnimation() { return( navigator.platform =3D=3D "Win32"= && navigator.appVersion.indexOf("Windows")>0 ) } function SupportsPPTHTML() { var appVer=3Dnavigator.appVersion, msie=3DappVer.indexOf("MSIE "), ver=3D0 if( msie >=3D 0 ) ver=3DparseFloat( appVer.substring( msie+5, appVer.indexOf(";",msie) ) ) else ver=3DparseInt(appVer) return( ver >=3D 4 && msie >=3D 0 ) } function _RSW() { if( !g_supportsPPTHTML || IsNts() || ( !g_scaleInFrame && (!IsWin("PPTSld") || !parent.IsFullScrMode()) ) ) return var padding=3D0; if( IsWin("PPTSld") && parent.IsFramesMode() ) padding=3D6 cltWidth=3Ddocument.body.clientWidth-padding cltHeight=3Ddocument.body.clientHeight-padding factor=3D(1.0*cltWidth)/g_origW if( cltHeight < g_origH*factor ) factor=3D(1.0*cltHeight)/g_origH newSize =3D g_origSz * factor if( newSize < 1 ) newSize=3D1 s=3DSlideObj.style s.fontSize=3DnewSize+"px" s.posWidth=3Dg_origW*factor s.posHeight=3Dg_origH*factor s.posLeft=3D(cltWidth-s.posWidth+padding)/2 s.posTop=3D(cltHeight-s.posHeight+padding)/2 if( g_scaleHyperlinks ) ScaleHyperlinks( factor ) } function _InitAnimations() { animRuntimeInstalled =3D ''+document.body.localTime !=3D 'undefined'; isFullScreen =3D (window.name =3D=3D "PPTSld") && !parent.IsFramesMode(); g_animUseRuntime =3D g_showAnimation && animRuntimeInstalled && !(isFullSc= reen && parent.IsSldVisited()); if( g_animUseRuntime ) { collSeq =3D document.all.tags("seq"); if( collSeq !=3D null ) { for(ii=3D0;ii numSlds ) gSldJumpIdx =3D numSlds; if ( gSldJumpIdx >=3D 0 ) { if ( gSldJumpIdx =3D=3D 0 ) gSldJumpIdx =3D 1; var jumpTo 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g_animInteractiveItems, g_animSlideTime; var g_animMainSequence =3D null; var ENDSHOW_MESG=3D"放映结束，单击= ;鼠标退出。", SCREEN_MODE=3D"Frames", gIsEndS= how=3D0, NUM_VIS_SLDS=3D38, SCRIPT_HREF=3D"script.js", FULLSCR_HREF=3D"full= screen.htm"; var gCurSld =3D gPrevSld =3D 1, g_offset =3D 0, gNtsOpen =3D gHasNts =3D gO= tlTxtExp =3D 0, gHasNarration =3D 0, gOtlOpen =3D true window.gPPTHTML=3DSupportsPPTHTML() var gMainDoc=3Dnew Array(new hrefList("slide0001.htm",1,-1,1),new hrefList(= "slide0003.htm",1,-1,1),new hrefList("slide0004.htm",1,-1,1),new hrefList("= slide0005.htm",1,-1,1),new hrefList("slide0006.htm",1,-1,1),new hrefList("s= lide0007.htm",1,-1,1),new hrefList("slide0008.htm",1,-1,1),new hrefList("sl= ide0039.htm",1,-1,1),new hrefList("slide0009.htm",1,-1,1),new hrefList("sli= de0010.htm",1,-1,1),new hrefList("slide0011.htm",1,-1,1),new hrefList("slid= e0038.htm",1,-1,1),new hrefList("slide0012.htm",1,-1,1),new hrefList("slide= 0043.htm",1,-1,1),new hrefList("slide0040.htm",1,-1,1),new hrefList("slide0= 041.htm",1,-1,1),new hrefList("slide0042.htm",1,-1,1),new hrefList("slide00= 15.htm",1,-1,1),new hrefList("slide0016.htm",1,-1,1),new hrefList("slide001= 7.htm",1,-1,1),new hrefList("slide0018.htm",1,-1,1),new hrefList("slide0019= .htm",1,-1,1),new hrefList("slide0020.htm",1,-1,1),new hrefList("slide0021.= htm",1,-1,1),new hrefList("slide0023.htm",1,-1,1),new hrefList("slide0022.h= tm",1,-1,1),new hrefList("slide0025.htm",1,-1,1),new hrefList("slide0024.ht= m",1,-1,1),new hrefList("slide0026.htm",1,-1,1),new hrefList("slide0044.htm= ",1,-1,1),new hrefList("slide0027.htm",1,-1,1),new hrefList("slide0046.htm"= ,1,-1,1),new hrefList("slide0028.htm",1,-1,1),new hrefList("slide0029.htm",= 1,-1,1),new hrefList("slide0032.htm",1,-1,1),new hrefList("slide0033.htm",1= ,-1,1),new hrefList("slide0035.htm",1,-1,1),new hrefList("slide0036.htm",1,= -1,1)); /********************************************* Frameset functions These functions control slide navigation and state of the frameset. **********************************************/ function FullScrInit() { g_allowAdvOnClick =3D GetCurSld().mAdvOnClk document.body.style.backgroundColor=3D"black" document.oncontextmenu=3Dparent._CM; document.onkeydown =3D _KDH; document.ondragstart=3DCancel document.onselectstart=3DCancel self.focus() } function Redirect( frmId ) {=09 var str=3Ddocument.location.hash,idx=3Dstr.indexOf('#'), sId=3DGetSldId() if(idx>=3D0) str=3Dstr.substr(1); if( window.name !=3D frmId && ( sId !=3D str) ) { obj =3D GetObj("Main-File") window.location.href=3Dobj.href+"#"+sId return 1 } return 0 } var MHTMLPrefix =3D CalculateMHTMLPrefix();=20 function CalculateMHTMLPrefix() { if ( document.location.protocol =3D=3D 'mhtml:') {=20 href=3Dnew String(document.location.href)=20 Start=3Dhref.indexOf('!')+1=20 End=3Dhref.lastIndexOf('/')+1=20 if (End < Start)=20 return href.substring(0, Start)=20 else=20 return href.substring(0, End)=20 } return ''; } function GetTags(base,tag) { if(g_supportsPPTHTML) return base.all.tags(tag); else return base.getElementsByTagName(tag); } function UpdNtsPane(){ if(frames["PPTNts"]) PPTNts.location.replace( MHTMLP= refix+GetHrefObj( gCurSld ).mNtsHref ) } function UpdNavPane( sldIndex ){ if(gNavLoaded) PPTNav.UpdNav() } function UpdOtNavPane(){ if(gOtlNavLoaded) PPTOtlNav.UpdOtlNav() } function UpdOtlPane(){ if(gOtlLoaded) PPTOtl.UpdOtl() } function SetHasNts( fVal ) { if( gHasNts !=3D fVal ) { gHasNts=3DfVal UpdNavPane() } } function ToggleOtlText() { gOtlTxtExp=3D!gOtlTxtExp UpdOtlPane() } function ClearMedia() { // Clear any sounds playing before launching another browser window. Other= wise, // in fullscreen mode, you'll continue to hear the sound in the frames mod= e. if (PPTSld.pptSound) PPTSld.pptSound.loop =3D 0; } function FullScreen() {=20 if ( PPTSld.g_animUseRuntime ) PPTSld.document.body.pause(); ClearMedia(); var href =3D ( document.location.protocol =3D=3D 'mhtml:') ? FULLSCR_HREF = : FULLSCR_HREF+"#"+GetHrefObj(gCurSld).mSldHref; if(PPTNav.event.ctrlKey) { var w =3D (window.screen.availWidth * 1.0) / 2.0 var h =3D w * (PPTSld.g_origH * 1.0) / PPTSld.g_origW win =3D window.open( MHTMLPrefix+href,null,"toolbar=3D0,resizable=3D1,top= =3D0,left=3D0," + "width=3D"+ w + ",height=3D" + h ); if( win.document.body && PPTSld.g_animUseRuntime ) win.document.body.PPTSldFrameset=3Dwindow; } else { win =3D window.open( MHTMLPrefix+href,null,"fullscreen=3Dyes" ); if( win.document.body && PPTSld.g_animUseRuntime ) win.document.body.PPTSldFrameset=3Dwindow; } } function ToggleVNarration() { rObj=3DPPTSld.document.all("NSPlay") if( rObj && !PPTSld.g_animUseRuntime ) { if( (rObj.playState =3D=3D 1)||(rObj.playState =3D=3D 0) ) rObj.Play() else if( rObj.playState =3D=3D 2 ) rObj.Pause() else return; } else if( PPTSld.g_animUseRuntime ) { narObj =3D PPTSld.document.all("narrationID") if( narObj ) narObj.togglePause() } } function GetCurSldNum() { =20 obj=3DGetHrefObj(gCurSld) if( obj.mOrigVis =3D=3D 1 ) return obj.mSldIdx else =20 return gCurSld } function GetNumSlds() { =20 if( GetHrefObj(gCurSld).mOrigVis =3D=3D 1 ) return GetSldList().mNumVisSlds; else return GetSldList().mList.length } function GetSldNum( href ) { for(ii=3D0; ii 1 ) PopSldList(); else if( !IsFramesMode() ) { if( gLoopCont ) GoToFirst() else EndShow() } } function GoToPrevSld() { ii=3DgCurSld-1 if( ii > 0 ) { obj=3DGetHrefObj(ii) while ( obj && ( obj.mVis =3D=3D 0 ) && ( ii>0 ) ) obj=3DGetHrefObj(--ii) if( ii =3D=3D 0 ) ii=3D1 GoToSldNum(ii) } } function GoToFirst(){ GoToSld( GetHrefObj(1).mSldHref ) } function GoToLast() { ii=3DGetSldList().mList.length if( ii !=3D gCurSld ) GoToSld( GetHrefObj(ii).mSldHref ) } function GoToSldNum( num ) { if( PPTSld.event ) PPTSld.event.cancelBubble=3Dtrue obj =3D GetHrefObj( num ) obj.mVis=3D1 gPrevSld=3DgCurSld gCurSld =3D num; PPTSld.location.replace(MHTMLPrefix+obj.mSldHref) if( IsFramesMode() ) { UpdNavPane(); UpdOtlPane(); UpdNtsPane() } } function GoToSld( href ) { if( PPTSld.event ) PPTSld.event.cancelBubble=3Dtrue GetHrefObj( GetSldNum(href) ).mVis=3D1 PPTSld.location.replace(MHTMLPrefix+href) } function SldUpdated( id ) { if( id =3D=3D GetHrefObj(gCurSld).mSldHref ) return gPrevSld=3DgCurSld gCurSld=3DGetSldNum(id) if( IsFramesMode() ) { UpdNavPane(); UpdOtlPane(); UpdNtsPane() } } function PrevSldViewed(){ GoToSld( GetHrefObj(gPrevSld).mSldHref ) } function HasPrevSld() { return ( gIsEndShow || ( gCurSld !=3D 1 && GetHrefO= bj( gCurSld-1 ).mVis =3D=3D 1 )||( GetCurSldNum() > 1 ) ) } function HasNextSld() { return (GetCurSldNum() !=3D GetNumSlds()) } function CloseWindow() { if( HideMenu() ) return; =09 var event =3D PPTSld.event; if( !IsFramesMode() && event && (event.keyCode=3D=3D27 || event.keyCode=3D= =3D32 || event.type=3D=3D"click" ) ) window.close( self ); CatchNumKeys( self, event ); } function Unload() { gIsEndShow=3D0; } function SetupEndShow() { gIsEndShow=3D1; PPTSld.document.body.scroll=3D"no"; PPTSld.document.onkeypress=3DCloseWindow; PPTSld.document.onclick=3DCloseWindow; PPTSld.document.oncontextmenu=3D_CM; } function EndShow() { if( IsFramesMode() ) return if( PPTSld.event ) PPTSld.event.cancelBubble=3Dtrue doc=3DPPTSld.document var dir =3D doc.body.dir if( dir !=3D "rtl" ) dir =3D "ltr"; doc.open() doc.writeln('


' + ENDSHOW_MESG + '

') doc.close() } function SetSldVisited(){ GetSldList().mList[gCurSld-1].mVisited=3Dtrue } function IsSldVisited(){ return GetSldList().mList[gCurSld-1].mVisited } function hrefList( sldHref, visible, advDelay, advClk ) { this.mSldHref=3D this.mNtsHref =3D sldHref this.mOrigVis=3D this.mVis =3D visible this.mVisited=3D false this.mAdvDelay=3D advDelay this.mAdvOnClk=3D advClk } function SldList(arr,curSld,fEnd) { this.mCurSld =3D curSld; this.mList =3D new Array(); var idx =3D 1; for(ii=3D0;ii 0) { PushSldList(sldList,fEnd); gCurSld =3D 1; } else if( PPTSld.event ) PPTSld.event.cancelBubble=3Dtrue } function PushSldList(arr,fEnd) { var ii =3D gSldStack.length; gSldStack[ii] =3D new SldList(arr,gCurSld,fEnd); GoToSld( gSldStack[ii].mList[0].mSldHref ); } function PopSldList() { if (gSldStack[gSldStack.length-1].fEndShow) EndShow() else { gCurSld =3D gSldStack[gSldStack.length-1].mCurSld; gSldStack[gSldStack.length-1] =3D null; gSldStack.length--; var sldList =3D gSldStack[gSldStack.length-1]; GoToSld( sldList.mList[gCurSld - 1].mSldHref ); } } var custShowList=3Dnew Array(); /********************************************* Navigation button implementation There are 2 types of buttons: ImgBtn, TxtBtn implemented as function objects. They share a similiar interface so the event handlers can call SetActive, for example, on a button=20 object without needing to know exactly=20 what type of button it is. **********************************************/ //---------------------------------- function ImgBtn( oId,bId,w,action ) //---------------------------------- { var t=3Dthis t.Perform =3D _IBP t.SetActive =3D _IBSetA t.SetInactive=3D _IBSetI t.SetPressed =3D _IBSetP t.SetDisabled=3D _IBSetD t.Enabled =3D _IBSetE t.ChangeIcon =3D null t.UserAction =3D action t.ChgState =3D _IBUI t.mObjId =3D oId t.mBorderId=3D bId t.mWidth =3D w t.mIsOn =3D t.mCurState =3D 0 } function _IBSetA() { if( this.mIsOn ) { obj=3Dthis.ChgState( gHiliteClr,gShadowClr,2 ) obj.style.posTop=3D0 } } function _IBSetI() { if( this.mIsOn ) { obj=3Dthis.ChgState( gFaceClr,gFaceClr,1 ) obj.style.posTop=3D0=20 } } function _IBSetP() { if( this.mIsOn ) { obj=3Dthis.ChgState( gShadowClr,gHiliteClr,2 ) obj.style.posLeft+=3D1; obj.style.posTop+=3D1 } } function _IBSetD() { =20 obj=3Dthis.ChgState( gFaceClr,gFaceClr,0 ) obj.style.posTop=3D0=20 } function _IBSetE( state ) { var t=3Dthis GetObj( t.mBorderId ).style.visibility=3D"visible" if( state !=3D t.mIsOn ) { t.mIsOn=3Dstate if( state ) t.SetInactive() else t.SetDisabled() } } function _IBP() { var t=3Dthis if( t.mIsOn ) { if( t.UserAction !=3D null ) t.UserAction() if( t.ChangeIcon ) { obj=3DGetObj(t.mObjId) if( t.ChangeIcon() ) obj.style.posLeft=3Dobj.style.posLeft+(t.mCurState-4)*t.mWidth else obj.style.posLeft=3Dobj.style.posLeft+(t.mCurState-0)*t.mWidth } t.SetActive() } =20 } function _IBUI( clr1,clr2,nextState ) { var t=3Dthis SetBorder( GetObj( t.mBorderId ),clr1,clr2 ) obj=3DGetObj( t.mObjId ) obj.style.posLeft=3Dobj.style.posLeft+(t.mCurState-nextState)*t.mWidth-obj= .style.posTop t.mCurState=3DnextState return obj } //----------------------------------------- function TxtBtn( oId,oeId,action,chkState ) //----------------------------------------- { var t=3Dthis t.Perform =3D _TBP t.SetActive =3D _TBSetA t.SetInactive=3D _TBSetI t.SetPressed =3D _TBSetP t.SetDisabled=3D _TBSetD t.SetEnabled =3D _TBSetE t.GetState =3D chkState t.UserAction =3D action t.ChgState =3D _TBUI t.mObjId =3D oId t.m_elementsId=3D oeId t.mIsOn =3D 1 } function _TBSetA() { var t=3Dthis if( t.mIsOn && !t.GetState() ) t.ChgState( gHiliteClr,gShadowClr,0,0 ) } function _TBSetI() { var t=3Dthis if( t.mIsOn && !t.GetState() ) t.ChgState( gFaceClr,gFaceClr,0,0 ) } function _TBSetP() { if( this.mIsOn ) this.ChgState( gShadowClr,gHiliteClr,1,1 ) } function _TBSetD() { =20 this.ChgState( gFaceClr,gFaceClr,0,0 ) this.mIsOn =3D 0 } function _TBSetE() { var t=3Dthis if( !t.GetState() ) t.ChgState( gFaceClr,gFaceClr,0,0 ) else t.ChgState( gShadowClr,gHiliteClr,1,1 ) t.mIsOn =3D 1 } function _TBP() { var t=3Dthis if( t.mIsOn ) {=20 if( t.UserAction !=3D null ) t.UserAction() if( !t.GetState ) return if( t.GetState() ) t.SetPressed() else t.SetActive() } =20 } function _TBUI( clr1,clr2,lOffset,tOffset ) { SetBorder( GetObj( this.mObjId ),clr1,clr2 ) Offset( GetObj( this.m_elementsId ),lOffset,tOffset ) } function Offset( obj, top, left ){ obj.style.top=3Dtop; obj.style.left=3Dle= ft } function SetBorder( obj, upperLeft, lowerRight ) { s=3Dobj.style; s.borderStyle =3D "solid" s.borderWidth =3D 1=20 s.borderLeftColor =3D s.borderTopColor =3D upperLeft s.borderBottomColor=3D s.borderRightColor =3D lowerRight } function GetBtnObj(){ return gBtnArr[window.event.srcElement.id] } function BtnOnOver(){ b=3DGetBtnObj(); if( b !=3D null ) b.SetActive() } function BtnOnDown(){ b=3DGetBtnObj(); if( b !=3D null ) b.SetPressed() } function BtnOnOut(){ b=3DGetBtnObj(); if( b !=3D null ) b.SetInactive() } function BtnOnUp() { b=3DGetBtnObj() if( b !=3D null ) b.Perform() else Upd() } function GetNtsState(){ return parent.gNtsOpen } function GetOtlState(){ return parent.gOtlOpen } function GetOtlTxtState(){ return parent.gOtlTxtExp } function NtsBtnSetFlag( fVal ) { s=3Ddocument.all.item( this.m_flagId ).style s.display=3D"none" if( fVal ) s.display=3D"" else s.display=3D"none" } function _BSetA_Border(){ b =3D gBtnArr[this.mObjId]; if( b !=3D null ) b.S= etActive() } function _BSetI_Border(){ b =3D gBtnArr[this.mObjId]; if( b !=3D null ) b.S= etInactive() } function _BSetP_Border(){ b =3D gBtnArr[this.mObjId]; if( b !=3D null ) b.S= etPressed() } function _BSetA_BorderImg() {=20 b =3D gBtnArr[this.mBorderId]=20 if( b !=3D null && this.mIsOn && !b.GetState() ) { obj=3Dthis.ChgState( gHiliteClr,gShadowClr,2 ) obj.style.posTop=3D0 } } function _BSetI_BorderImg() {=20 b =3D gBtnArr[this.mBorderId] if( b !=3D null && this.mIsOn && !b.GetState() ) { obj=3Dthis.ChgState( gFaceClr,gFaceClr,1 ) obj.style.posTop=3D0 } } var gHiliteClr=3D"THREEDHIGHLIGHT",gShadowClr=3D"THREEDSHADOW",gFaceClr=3D"= THREEDFACE" var gBtnArr =3D new Array() gBtnArr["nb_otl"] =3D new TxtBtn( "nb_otl","nb_otlElem",parent.ToggleOtlPan= e,GetOtlState ) gBtnArr["nb_otlElem"] =3D new TxtBtn( "nb_otl","nb_otlElem",parent.ToggleOt= lPane,GetOtlState ) gBtnArr["nb_nts"] =3D new ImgBtn( "nb_nts","nb_ntsBorder",10,parent.ToggleN= tsPane ) gBtnArr["nb_nts"].SetActive =3D _BSetA_BorderImg; gBtnArr["nb_nts"].SetInactive =3D _BSetI_BorderImg; gBtnArr["nb_ntsBorder"] =3D new TxtBtn( "nb_ntsBorder","nb_ntsElem",parent.= ToggleNtsPane,GetNtsState ) gBtnArr["nb_ntsElem"] =3D new TxtBtn( "nb_ntsBorder","nb_ntsElem",parent.To= ggleNtsPane,GetNtsState ) gBtnArr["nb_prevBorder"] =3D gBtnArr["nb_prev"]=3D new ImgBtn( "nb_prev","n= b_prevBorder",30,parent.GoToPrevSld ) gBtnArr["nb_nextBorder"] =3D gBtnArr["nb_next"]=3D new ImgBtn( "nb_next","n= b_nextBorder",30,parent.GoToNextSld ) gBtnArr["nb_sldshw"]=3D new ImgBtn( "nb_sldshw","nb_sldshwBorder",18,parent= .FullScreen ) gBtnArr["nb_sldshwBorder"] =3D new TxtBtn( "nb_sldshw","nb_sldshwBorder",pa= rent.FullScreen,null ) gBtnArr["nb_sldshwBorder"].SetActive =3D _BSetA_Border; gBtnArr["nb_sldshwBorder"].SetInactive =3D _BSetI_Border; gBtnArr["nb_sldshwText"] =3D new TxtBtn( "nb_sldshw","nb_sldshwText",parent= .FullScreen,null ) gBtnArr["nb_sldshwText"].SetActive =3D _BSetA_Border; gBtnArr["nb_sldshwText"].SetInactive =3D _BSetI_Border; gBtnArr["nb_voice"] =3D gBtnArr["nb_voiceBorder"] =3D new ImgBtn( "nb_voice= ","nb_voiceBorder",18,parent.ToggleVNarration ) gBtnArr["nb_otlTxtBorder"] =3D gBtnArr["nb_otlTxt"]=3D new ImgBtn( "nb_otlT= xt","nb_otlTxtBorder",23,parent.ToggleOtlText ) gBtnArr["nb_ntsBorder"].m_flagId=3D "nb_nts" gBtnArr["nb_ntsBorder"].SetFlag =3D NtsBtnSetFlag gBtnArr["nb_otlTxt"].ChangeIcon=3D GetOtlTxtState /********************************************* Context menu implementation _CM() is the function that's hooked up to the oncontextmenu event. Once we're asked to show the menu, we first build it by creating DIVs on-the-fly. Then we position it=20 within the screen area so it doesn't get clipped. Creating the DIVs using createElement() means we don't have to write out any extra HTML into the slide HTML files. **********************************************/ var sNext=3D"下一步",sPrev=3D"上一步",s= End=3D"结束放映",sFont=3D"宋体",sArrow= =3D"箭头",sFreeform=3D"任意形状",sRect= =3D"矩形",sOval=3D"椭圆" function ShowMenu() { BuildMenu(); var doc=3DPPTSld.document.body,x=3DPPTSld.event.clientX+doc.scrollLeft,y= =3DPPTSld.event.clientY+doc.scrollTop m =3D PPTSld.document.all.item("ctxtmenu") m.style.pixelLeft=3Dx if( (x+m.scrollWidth > doc.clientWidth)&&(x-m.scrollWidth > 0) ) m.style.pixelLeft=3Dx-m.scrollWidth m.style.pixelTop=3Dy if( (y+m.scrollHeight > doc.clientHeight)&&(y-m.scrollHeight > 0) ) m.style.pixelTop=3Dy-m.scrollHeight m.style.display=3D"" } function _CM() { if( !parent.IsFullScrMode() ) return; if(!PPTSld.event.ctrlKey) { ShowMenu() return false } else HideMenu() } function BuildMenu() { if( PPTSld.document.all.item("ctxtmenu") ) return var mObj=3DCreateItem( PPTSld.document.body ) mObj.id=3D"ctxtmenu" mObj.style.visibility=3D"hidden" var s=3DmObj.style s.position=3D"absolute" s.cursor=3D"default" s.width=3D"120px" SetCMBorder(mObj,"menu","black") var iObj=3DCreateItem( mObj ) SetCMBorder( iObj, "threedhighlight","threedshadow" ) iObj.style.padding=3D2 CreateMenuItem( iObj,sNext,M_GoNextSld,M_True ) CreateMenuItem( iObj,sPrev,M_GoPrevSld,M_HasPrevSld ) =09 CreateSeparator( iObj ) CreateMenuItem( iObj,sEnd,M_End,M_True ) mObj.style.visibility=3D"visible" } function Cancel() { window.event.cancelBubble=3Dtrue; window.event.returnVa= lue=3Dfalse } function Highlight() { ChangeClr("activecaption","threedhighlight") } function Deselect() { ChangeClr("threedface","menutext") } function Perform() { e=3DPPTSld.event.srcElement if( e.type=3D=3D"menuitem" && e.IsActive() ) e.Action() else PPTSld.event.cancelBubble=3Dtrue } function ChangeClr( bg,clr ) { e=3DPPTSld.event.srcElement if( e.type=3D=3D"menuitem" && e.IsActive() ) { e.style.backgroundColor=3Dbg e.style.color=3Dclr } } function M_HasPrevSld() { return( parent.HasPrevSld() ) } function M_GoNextSld() { if( gIsEndShow ) M_End(); else GoToNextSld() } function M_GoPrevSld() { if( gIsEndShow ) { gIsEndShow=3D0; history.back();= PPTSld.event.cancelBubble=3Dtrue; } else GoToPrevSld() } function M_True() { return true } function M_End() { window.close( self ) } function CreateMenuItem( node,text,action,eval ) { var e=3DCreateItem( node ) e.type=3D"menuitem" e.Action=3Daction e.IsActive=3Deval e.innerHTML=3Dtext if( !e.IsActive() ) e.style.color=3D"threedshadow" e.onclick=3DPerform e.onmouseover=3DHighlight e.onmouseout=3DDeselect s=3De.style; s.fontFamily=3DsFont s.fontSize=3D"9pt" s.paddingLeft=3D2 } function CreateSeparator( node ) { var sObj=3DCreateItem( node ) SetCMBorder(sObj,"menu","menu") var s=3DsObj.style s.borderTopColor=3D"threedshadow" s.borderBottomColor=3D"threedhighlight" s.height=3D1 s.fontSize=3D"0px" } function CreateItem( node ) { var elem=3DPPTSld.document.createElement("DIV") node.insertBefore( elem ) return elem } function SetCMBorder( o,ltClr,rbClr ) { var s=3Do.style s.backgroundColor=3D"menu" s.borderStyle=3D"solid" s.borderWidth=3D1 s.borderColor=3DltClr+" "+rbClr+" "+rbClr+" "+ltClr } ------=_NextPart_01C48438.43A1D1D0 Content-Location: file:///C:/85899A73/23.files/fullscreen.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii"
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