MIME-Version: 1.0 Content-Type: multipart/related; boundary="----=_NextPart_01C48431.FDF062B0" 此文档为“单个文件网页”，也称为“Web 档案”文件。如果您看到此消息，但是您的浏览器或编辑器不支持“Web 档案”文件。请下载支持“Web 档案”的浏览器，如 Microsoft Internet Explorer。 ------=_NextPart_01C48431.FDF062B0 Content-Location: file:///C:/85899A55/15.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" PowerPoint 演示文稿

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单击此处编辑母版= 6631;题样式
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第十五章=
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基因重组&#= 19982;基因工程 =
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ò= 07;言 <= /span>
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     21世纪是生命科学的世纪！<= /span> =
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   生物技术<= /span>是当今发展最= 快、最活跃、 对社会<= span style=3D'font-family:SimHei;mso-ascii-font-family:SimHei;mso-fareast-font-= family: SimHei;font-size:117%'>中基因工程和发酵工程是生物技术的= 核心。
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     世界各国政府高度= 7325;视生物技术<= b>
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   我国政府将其= 列为信息通讯、航天= 2289;新能源、  <= span style=3D'font-family:SimHei;mso-ascii-font-family:SimHei;mso-fareast-font-= family: SimHei;font-size:117%'>新材料等高科= 技领域的第二位。可见其战略= 地位。<= b>
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     生物技术正在改变人们௚= 0;生活和观念
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     随着基因组计划= 的接近完成及= 后基因组&#= 35745;划的开展，借助计= 算机处理复杂信息的= 4378;大能力，21世= 纪的生物技术将会有更大发= 展，有些领域甚至可= 3021;= 有重大突破。= =
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第一节  = 自然界的基因转= 移和重组
一、接合
二、转化
三、转导
四、转座
质粒DNA从一个细胞转移至另= ;一细胞
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   = 病毒把某一细胞的DNA带至另一细胞，
使后者获得新的遗传= ;表型
DNA片段从染色体= 的某处移位到另一处= 2290;
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• 重&#= 32452;的类型：
1.位点特异性重组
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•2. 同源重组
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四、基因重组(recombinant)
不同DNA分子= 间发生的共价连接=
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同源区<= /b>
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机&#= 21046;
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进行交= 换，形成生物个（群= 5289;体的多样性。
2.&nb= sp; 是DNA损伤修复的重要机制之一。
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•2. 位点特异性的基因重&#= 32452;
•  
•      由整合酶催化，= 重组仅在特定= 的基因片段和位点= 上进行。
•
•     是免疫球蛋&#= 30333;多样性的分子= 机制。
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=
=
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第二节
=
=
=
基因= 4037;程的工具
= 212;—工具酶和载Ë= 07;= 3;
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一、 常用的工具酶
• 
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(一)限= 1046;性内切酶(restriction endonuclease)
  ---切——基因&= #24037;程的手术刀 =
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功能： =
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     识别双链DNA中特异序列，该序&= #21015;的特征为= 4-6个核&#= 33527;酸的回文结构，并在识别序= 列内或附= 近特异切割DNA，产&#= 29983;粘性末端或平末端。
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  &nbs= p; 根&#= 25454;需要在特定的位点Ŀ= 34;确切割
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   没有= 限制性内&#= 20999;酶的发现和&#= 24212;用，就= 很难有今&#= 22825;分子生物学与基因ð= 37;程的快= 速发展。
• 
=
=
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作用= 模式图：
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• 
=
=
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作用= 模式图：
=
=
=
2.  产生3′= ;突出粘性末端
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作用= 模式图：
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限制性内= 0999;酶用= 途：<= /div>
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1.  因片段，切割载体= 4418;成切口，使目的基因能插入载= 体中。
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2.限制= 4615;片段长度多态性= (Restriction = 3;
    Fragment Length Polymorphysms<= b>,  <= /div>
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  = DNA指纹分析等。 <= /div>
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3.构建DNA= 的物理图谱，基因定= 0301;，DNA的
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------=_NextPart_01C48431.FDF062B0 Content-Location: file:///C:/85899A55/15.files/slide0010.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" PowerPoint 演示文稿
(二)DNA连接酶(DNA ligase)
         ——基因工程的缝纫= 8024; <= /div>
• 
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=
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     将两条双链= DNA片段连接起来，= 实现 DNA的体外重组。
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功能： =
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      催化两条双链= DNA分子的互补粘性末<= span style=3D'font-family:SimHei;mso-fareast-font-family:SimHei;font-size:150%; color:#3366FF'>端或平末端的5′磷酸基团与3′羟基形= 成磷酸酯键。
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=
=
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       也可将双链DNA分子内部的单&= #20010;切= 口= (无核苷酸空缺= )连接起来。还可作用= ;<= span style=3D'font-family:SimHei;mso-fareast-font-family:SimHei;font-size:150%'= >于= RNA，但效率很低。
=
=
=
       如需连接单链= DNA或= RNA，则需用<= span lang=3DEN-US style=3D'font-family:"Times New Roman";mso-ascii-font-family:= "Times New Roman"; mso-fareast-font-family:SimHei;font-size:150%;color:#FF3300;mso-fareast-la= nguage: ZH-CN'>RNA连接酶。
=
=
=
DNA连接酶催化平末端连接&= #35201;比粘性末= 端 效率低得多。= <= /span>
------=_NextPart_01C48431.FDF062B0 Content-Location: file:///C:/85899A55/15.files/slide0012.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" PowerPoint 演示文稿
DNA连接酶
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DNA连接酶
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作用模式图：
=
=
=
= 1.  连接粘性末端
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作用模式图：  = 2. 连接平&#= 26411;端 <= /div>
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DNA连接酶
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(三) 反转录酶<= /span>(Reverse Transcriptase)
•
=
=
=
功能：
=
=
=
       以RNA为模板，以带= = 3′= -OH末端的= DNA片段为引物， 沿= 5′至= 3′方向×= 12;成= DNA链。
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反转录酶
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5′= TTTTTTT—OH  3′
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AGACAGGAT……= 3′=
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= 1.   RNA指导的DNA聚合酶活&#= 24615;：
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5′= TTTTTTT
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• <= /div>
=
=
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2. DNA指导的DNA聚合酶活&#= 24615;；核糖核<= span style=3D'font-family:SimHei;mso-fareast-font-family:SimHei;font-size:150%; color:#3366FF'>酸酶H活性；DNA解旋酶活&#= 24615;等。
=
=
=
用途：
=
=
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= 1. 反转录酶的最主= ;要作用是以mRNA
为模板合成互补DNA(complementary
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=
=
=
= 2. 以单链= DNA或= RNA为模板制备探针。
=
=
=
功能：
------=_NextPart_01C48431.FDF062B0 Content-Location: file:///C:/85899A55/15.files/slide0016.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" PowerPoint 演示文稿
(四= )  核酸酶S1
• <= /div>
=
=
=
功能： =
=
=
=
   水解双链DNA、RNA或DNA-RNA杂交体= 中的单链部分。
=
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核酸酶S1
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核酸酶S1
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核酸酶S1
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(四)&#= 26680;酸酶S1
•
=
=
=
用途：
=
=
1.  除去<= /b>双链DNA的粘&#= 24615;末端以产生
    平末端；
=
=
=
= 2.  除去<= /b>= cDNA合成时形成的发夹结构；
=
=
3. 分析= RNA的茎环结构以及= DNA-
    RNA分子的杂交情况等。= ;
------=_NextPart_01C48431.FDF062B0 Content-Location: file:///C:/85899A55/15.files/slide0018.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" PowerPoint 演示文稿
• 
=
=
=
功能：
=
=
=
催化脱&#= 27687;核糖核苷酸转移到单链或双链= DNA分子的= 3′= -OH末端上。
=
=
1.底物是单链DNA或有3′= 突出末端的
    双链 DNA。
=
=
=
<= b>OH
=
=
=
5′
=
=
=
3′
=
=
=
Mg2+
=
=
=
<= b>dNTP
=
=
=
5′
=
=
=
3′
=
=
=
(<= b>A= 、<= b>G= 、<= b>C= 、<= b>T)n
=
=
=
n= ppi
=
=
=
=
=
=
5′
=
=
=
3′
=
=
=
<= b>OH
=
=
=
Mg2+
=
=
=
<= b>dNTP
=
=
=
n= ppi
=
=
=
5′
=
=
=
3′
=
=
=
(<= b>A= 、<= b>G= 、<= b>C= 、<= b>T)n
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Content-Location: file:///C:/85899A55/15.files/slide0020.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" PowerPoint 演示文稿
• 
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  = 9992;途：
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 1.  主要作用是在= 载体或目的基因 3′= 末端加上同源多聚尾巴，形成人工粘= 性末端，便&= #20110;= DNA重组。
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= 2.  DNA 3′末端的<= span style=3D'font-family:SimHei;mso-fareast-font-family:SimHei;font-size:150%; color:#FF3300'>同位素标记。
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(六) 核糖核酸酶H
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水解DNA-RNA杂交分子中的= RNA链。
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RNA
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DNA
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核糖核酸酶H
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DNA
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用途：
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功能：
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 用核糖核酸酶H部分水解= mRNA-= = cDNA杂交分子中的= mRNA，使未= 被水解的= mRNA作为引物合成= = cDNA的第二链。
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二、载体(vector)
• 
=
=
=
<= span style=3D'font-size:133%;color:#003CFC'>*目的基因进入原&#= 26680;或真核细胞并高效<= /span>复制=
   和表&#= 36798;蛋白质，需要装入载体才&#= 33021;实现。
=
=
=
<= span style=3D'font-size:150%;color:#FF3300'>*=  作为&= #22522;因工程载体所需具= 791;的基本条件： <= /span>
=
=
=
= 1.  能自我复制，并具一定的拷贝数= ;。
=
=
=
= 2.  带有<= /b>抗性基因，便于筛选和鉴定。= ;
=
=
4.  带有<= /b>强启动子，驱动目的基因在宿= ;主
    细胞内高效表达。
=
=
3.  在适&= #24403;的位置有单酶切位点，便于重
    组操作。
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• 
=
=
1.  ಭ= 4;菌质粒：最常用的用于= 0446;的基因克隆或
        = ;      表达的载体。
=
=
2.  l噬菌体：<= /span> 主要= 992;于构建基因组= DNA或
        = ;     cDNA文库。
=
=
3.  M13ࢤ= 4;菌体：专用于= Sanger双脱氧= DNA
        = ;        测序
=
=
=
4.  粘ľ= 90;：用于克&#= 38534;大片段DNA。 =
=
=
=
5.  酵ē= 97;质粒：用于在= 7237;母中表达目的蛋白。
=
=
=
6.  病毒：<= span style=3D'font-family:SimHei;mso-fareast-font-family:SimHei;font-size:133%'= >包括人或哺乳动物病&#= 27602;、昆虫病毒及= 植物病毒。用于在真核细胞中表达目的蛋白。
基因工程๙= 3;体的种类和用途：
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(一) 质粒(= plasmid)
• 
<= /span>
=
=
=
=
细菌培养
=
=
=
质粒扩增并表达= 4507;白质
=
=
=
大肠杆菌
=
=
=
染色质
=
=
=
质粒
定&#= 20041;:细菌染色质= 0197;外的双链环状= DNA，
能自我复制= 1644;表达其携带的遗传߼= 9;息。&= #13;
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Ampr
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ORI
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Tetr
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pBR322质粒：一种&= #20811;隆质粒 = ;
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ORI：复制起始点= 5292;
保证高= 335;贝自我复制。 =
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结构：
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Ampr, <= span lang=3DEN-US style=3D'font-family:"Times New Roman";mso-ascii-font-family:= "Times New Roman"; mso-fareast-font-family:SimHei;font-size:117%;color:#FF3300;mso-fareast-la= nguage: ZH-CN'>Tetr：
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两个抗性基= 2240;用于
筛选阳性克隆。
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Pst<= /span> I,  BamH I<= span style=3D'font-family:SimHei;mso-fareast-font-family:SimHei;font-size:117%; color:#FF3300'>：
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两个单%= 238;切位点用于= 插入目的基因
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------=_NextPart_01C48431.FDF062B0 Content-Location: file:///C:/85899A55/15.files/slide0045.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" PowerPoint 演示文稿
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LacZ
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MCS
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pfxBlue: 克隆质粒
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MCS：Multiple Clonning Site
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LacZ: 蓝白斑筛选阳= 4615;克隆
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P(LacZ)
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MCS
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Ampr&#= 13;
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LacZ
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pRS426ᦂ= 6;原核细胞表达= 6136;粒
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P(LacZ)： = LacZ启动子
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用于在原核细胞驱动目的= 基因的表达。
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pcDNA3ᦂ= 6;真核细胞表达= 6136;粒
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Pcmvᦂ= 6; =
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CMVࣜ= 6;强子/启动子
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  驱动目的<= /span>= 基因在真核= 细胞内表达
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BGH pAᦂ= 6;加尾信号
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 目的基因&#= 36716;录后加上polyA= 3614;，使其更稳定。
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插入型
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=
=
置换型
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=
=
=
=
=
=
=
筛选标记： 蓝白斑= (LacZ)、噬&#= 33740;斑等。
=
=
(1) 重组噬菌体的分子量必= 须在野生型噬 <= /div>
     菌体 分子量大小= 的75%至105%之间&#= 12290; <= /div>
=
=
=
用途：
=
=
=
= b.用于构建基因组= 或cDNA文库。
=
=
=
= c.用于抗体库或随= 机肽库的构建。
a.用&#= 20316;一般的克隆载体。<= /span>
------=_NextPart_01C48431.FDF062B0 Content-Location: file:///C:/85899A55/15.files/slide0051.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" PowerPoint 演示文稿
2. 粘粒(cosmid)
• 
=
=
=
组成：
=
=
=
= 2.  抗性&= #22522;因；
=
=
=
= 1.  质粒&= #22797;制的起始位点= (ORI)；
=
=
=
= 3.  用于&= #25554;入目的基因的单个%= 238;切位点；
=
=
=
= 4. = l噬菌体的cos位点。 <= /div>
=
=
=
      可见，cosmid是由质粒&#= 21644;l= 2124;菌体的= cos位点构建而成。
------=_NextPart_01C48431.FDF062B0 Content-Location: file:///C:/85899A55/15.files/slide0052.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" PowerPoint 演示文稿
• 
=
=
=
特点：
=
=
1.可插入= 长达27～41kb的外源基因，= 方便地用于克隆大片段DNA。
=
=
2.加入l噬菌体头部和尾部蛋白，可= ;将
    粘粒包装成类似于l= 2124;菌体的具感染能力的颗粒，方便地= ;进入大肠杆菌。=
=
=
3.粘粒进入细菌= 后则完全失去噬菌体
    的功能，而表现质粒的特性= 。
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3.  M13噬&= #33740;体 <= /div>
• &nbs= p;
=
=
=
噬菌体
正链
=
=
=
复制
=
=
=
复制型
DNA<= span lang=3DEN-US style=3D'font-family:SimHei;mso-fareast-font-family:SimHei; font-size:88%;mso-fareast-language:ZH-CN;mso-special-format:lastCR;display: none'>
=
=
=
经pII蛋白
造缺口=
=
=
=
3′<= /b>
=
=
=
5′<= /b>
=
=
=
经pII
蛋白
剪切
=
=
=
释出单链DNA(正链= )
=
=
=
进入下
一循环
=
=
=
5′<= /b>
=
=
=
3′<= /b>
=
=
=
3′<= /b>
=
=
=
5′<= /b>
=
=
=
滚环复制
在细菌内的复制模式= ;图
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• &nbs= p;
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 M13噬= ;菌体几个重要特点：
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1.  M13= 2124;菌体不溶解= 3487;主细胞，只是降
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2.  M13噬菌体Ŋ= 21;以单໦= 2;和双໦= 2;两మ= 1;方式存ࢷ= 2;，因此可以用感੡= 9;(经= ;包装的单链DNA)和转化(双໦= 2;DNA)。
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3. 克隆的外&#= 28304;基因片段不宜大于<= /span>1000bp。
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•
=
=
=
M13噬= ;菌体用途：
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1.  DNA<= span style=3D'font-family:SimHei;mso-fareast-font-family:SimHei;font-size:167%; color:#0066FF'>序列测定；
=
=
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2.  制备单链DNA探针；
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=
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3.  用于定点突变的= 研究。
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=
=
=
第三节&#= 13;
=
=
=
基因工程的= 7493;骤&#= 13;
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=
=
=
基因工程į= 40;基本步骤
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1.基因ॣ= 7;程的上游技术：基因重组
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=
+
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=
=
+
=
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连接 =
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转化、筛选鉴定阳性克隆<= span style=3D'font-family:SimHei;mso-ascii-font-family:SimHei;mso-fareast-font-= family: SimHei;font-size:117%;color:#669900;mso-special-format:lastCR;display:none= '>
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=
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含重组子阳性克隆&= #13;
=
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载体
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目的基因
分、切、接、转= 2289;筛
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charset="us-ascii" PowerPoint 演示文稿
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        发酵条件= (生物反应器结构、= 细菌培养温度、空气&#= 36890;量、= pH及= 培养基成分等= )的优化组合。
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基因工程į= 40;基本步骤
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一 = 289; 目的基因的制备= —分 <= /div>
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(一<= b>) 人工合成法= (也即化学合成法<= span lang=3DEN-US style=3D'font-family:"Times New Roman";mso-ascii-font-family:= "Times New Roman"; mso-fareast-font-family:SimHei;font-size:150%;color:black;mso-color-index: 3;mso-fareast-language:ZH-CN'>)
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      本方法适用于分子量较小的目的= 基因 (100bp以内= )。
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缺点：
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 2.  如目的基因= DNA序列需通过氨基酸序
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1. 只能合成较小片段的= ;= DNA(100bp以= 内= )。
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=
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(二= ;)反转录法 =
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     本法是应用得最多的获取目的= 基因的方法。= 但前提是目的基因的= <= span style=3D'font-family:SimHei;mso-ascii-font-family:SimHei;mso-fareast-font-= family: SimHei;font-size:167%'>序列已知，至= 少部分已知。
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(二)反转录法：
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方案：
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T= TnT=   5′
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c= DNA
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核糖= 6680;酸酶H
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T= TnT=
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DNA = poly I
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c= DNA
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双链cDNA
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DNA聚ࡧ= 2;酶
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扩增出大量的产物
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file:///C:/85899A55/15.files/slide0060.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" PowerPoint 演示文稿
•
=
=
=
(三)直接从= 染色体DNA中分离=
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=
=
+  本方&= #27861;较适用于制备原核生物目的基= 因，其原因为：
=
=
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 = 1.  真核&= #32454;胞染色体= DNA有丰富的内含 
      子，在原= 核细胞中转录后不能= 1098;= 接。    
=
=
=
 2.  真核细胞的基= 因多数为单拷贝，难<= /b> 
      于分离到足够的目的= ;基因。
        根据染色体= DNA的限制性内切酶图= = 谱，用合= 适的限制性内切酶切= 1106;染色体= = DNA，分离目的基因。
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• 
=
=
=
(四) 从基因文库(gene library)中获取=
=
=
=
基因组文库：G-文库
=
=
=
= cDNA文库： c-文库
   (常用)
=
=
=
方法：
=
=
=
基因文库
=
=
=
      用目的基因上的一段= ;
DNA做成探针与文库= 中的
DNA作核酸杂交，从基因
文库中“钓出= 221;有目的基
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(二)平末端连接
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产生平末端的 =
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产生粘性末= 471;&= #13;
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核酸酶= S1
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目的基因
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产生= 粘性末端&= #13;
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核酸酶= S1
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(三)用接头连接
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三、将重组体(子)导入宿主ń= 54;胞---转 <= /div>
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1. 重组质粒的导入方法
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大肠杆菌
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CaCl2
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0～4℃
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感受态细胞
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重组质粒
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   转化
transformation=
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含重ń= 52;质粒的大肠杆菌
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三、将重组体导= 入宿主细胞
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2. 重组病毒或&#= 37325;组噬菌体= 0340;导入方法
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重组病&#= 27602;
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重组噬&#= 33740;体
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加入病毒į= 40;
某些蛋白ń= 52;分
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具有感染能力的病毒或噬菌体颗粒<= /b>
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真核细胞
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原核细胞
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=
转染= (transfection)
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例1：
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X-gal
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蓝色化合物
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b-半乳糖苷&#= 37238;
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例2：蓝白斑筛选
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2.  菌落原位杂交
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覆盖
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4  5  6
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PowerPoint 演示文稿
=
=
=
 五、基因工程的= 下游技术
=
=
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(ߌ= 8;)  细菌发酵 =
=
=
=
(ߚ= 8;)  蛋白质的分离ӌ= 9;纯化与质量鉴定
=
=
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  <= /b>发酵条件<= /span>(生物= 453;应器的结构、细菌
培养的= 温度、空气的通量、pH&= #21450;培养基
成分等= )的优化组×= 12;。 =
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&= #20845;、基因工程中几个= 840;手的问题
• 
=
=
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<= span style=3D'font-size:150%'>Ø= = 1. 原核细= ;胞的RNA聚合酶不能识别
     真核细胞௚= 0;启动子。
=
=
=
<= span style=3D'font-size:150%'>Ø= 2. 原核细= ;胞不能剪接真核细胞 <= /b>
     带内含子的mRNA。
=
=
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Ø= 3. 原核细= ;胞表达出的真核细胞ൺ= 7;白质
   &= nbsp; 往往= 6080;活性。
=
=
=
= Ø= 4. 用真核细胞表达系统= 虽然可以表达= 有活性的蛋&= #30333;质，但其产量太低，成本= 2826;高。 <= /span>
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=
=
=
  基因工程在Õ= 07;学上的应用
 
 
=
=
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<= span style=3D'font-family:SimHei;font-size:133%;color:#FF3300'>v  现代医学越来= 越重视活性蛋白&#= 36136;在预防 <= /div>
   和治疗疾病中的作= 9992;。
=
=
=
<= span style=3D'font-family:SimHei;font-size:133%;color:#FF3300'>v  基因工程可大量生产天然稀有&#= 23384;在的
   重要的肽或蛋白&#= 36136;。 <= /div>
=
=
=
<= span style=3D'font-family:SimHei;font-size:133%;color:#FF3300'>v  基因工程可根据人的意= 愿设计生产出
   天然不存在的有重= 5201;生物活性的肽或
   蛋白质= 2290;
=
=
=
<= span style=3D'font-family:SimHei;font-size:133%;color:#FF3300'>v  人类基因组计= 1010;及后基因组计划的成果 <= /div>
   将为= 基因工程提供难于估量= 的广阔发展
   空间。
一、基因工程生= 0135;的药品
•
=
=
=
1.基因工程ĭ= 23;苗 =
=
=
=
   乙肝，丙肝，爱滋= 0149;，流行性感冒，= = 出血热，人乳= 2836;瘤病毒，巨细胞病ૈ= 2;= 等。
=
=
=
   结核，麻风，霍乱= 5292;痢疾，肺炎等。
=
=
=
   血吸虫，疟疾等。
=
=
=
   各种肿瘤疫苗。
=
=
=
……=
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• <= /div>
=
=
=
= 2.  = 0154;体活性因子
=
=
=
<= span style=3D'font-size:150%;color:#CC00FF'>Ø  = 4178;扰素(IFN)：
=
=
=
<= span style=3D'font-size:150%;color:#CC00FF'>Ø  = 0333;细胞介素(IL)：
=
=
=
= Ø<= /span>  = 9983;长因子：
=
=
=
Ø  <= /span>集落刺激 = 因子(CSF)：
=
=
=
= IFN-a，= IFN-b，= IFN-g。
=
=
=
= IL-1，= 2，= 3……13。
=
=
=
 神经&= #29983;长因子，表皮生长= = 因子，成纤维细胞生= ;长因= 子等。
=
=
=
干细胞= -CSF，粒细胞= -= = CSF，粒细胞= -巨噬细胞= -= = CSF。
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• 
=
=
=
2. 人体活性&#= 22240;子
=
=
=
        红细胞生成素= (EPO)，超氧化物歧= 化酶= (SOD)，组织纤溶酶原激活= ;物= (tPA)= 等。 <= /span>
=
=
=
= v<= /span>   激素
=
=
=
…… = ;
=
=
=
<= span style=3D'font-size:150%;color:#CC00FF'>v   治疗心血管及血= 液病的活性肽
=
=
=
胰岛素，生长激素，= ;生长抑制激素等。
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• 
=
=
=
v<= /span>  主要发展趋势<= /div>
=
=
=
Ø  完善现有的原= 6680;和真核细胞表达体౿= 5;
=
=
=
<= span style=3D'font-family:SimHei;font-size:150%;color:#CC00FF'>Ø  建立新的表达= 0307;系
=
=
=
Ø  改进和发展大= 5268;模动、植物细胞培= 养技术
=
=
=
q 应用转基因动、植物&#= 20316;为生物反应&#= 22120;= 高产量、&#= 39640;质量、低成本生产×= 17;贵生物= 药物。     
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二、 基= 240;诊断 <= /div>
• 
=
=
=
<= span style=3D'font-size:150%;color:#CC00FF'>q   基因诊断的概念
=
=
=
<= span style=3D'font-size:150%;color:#CC00FF'>q   基因诊断所应用= 的主要技术
=
=
=
Ø= ;  = 2858;合酶链反应= (PCR)
=
=
=
    将痕量的= 难于检测到的特定的基因片段成= 百万地特异地扩增。
=
=
=
&Oslas= h;  = 6680;酸探针技术
=
=
=
=
=
=
=
       用已知的与疾病相关= ;或特异的核酸片段= = (探针= )与待测样品作核酸= 杂交，检测= 样品是否= 有该特征性DNA片段以及量的= 多少。
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̶= 6;Southern Blot:     = 印迹DNA <= /div>
̶= 6;Northern Blot:     = 印迹RNA <= /div>
̶= 6;原位杂交技术
̶= 6;寡核苷酸探针技= 6415;
̶= 6;RT-PCR
̶= 6;PCR-单链构象多态分= 6512;(PCR-SSCP)
核酸探针&#= 25216;术
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=
=
=
<= span style=3D'font-size:150%;color:#CC00FF'>q  = 2522;因诊断的疾病谱：
=
=
=
         遗传性疾病，= 感染性疾病，心血管= 疾病，肿瘤，= ...
=
=
=
q  = 1457;现并获得某种疾病௚= 0;特异性= 基因是基因诊断的基础= 。
=
=
=
  人类&= #22522;因组计划，后基因 = 452;计划，= 特别是疾病基因组计划= 将极大地推= 动这一进程。
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三、基因= 835;疗(gene therapy)
• <= /div>
=
=
=
<= span style=3D'font-family:SimHei;font-size:150%;color:#CC00FF'>v 基因治疗的策略 =
=
=
=
Ø1.<= /span>基因置换：以正常基因替换缺陷基因。
=
=
=
 一切通过&#= 22522;因水平的操作而达Ò= 40;，
  治疗疾病目的疗法= 2290;
=
=
=
Ø2.<= /span>基因补偿：导入正常基= 因，补偿缺    = 陷基因的功能。=
=
=
=
Ø3.<= /span>基因失活&#= 65306;用反义核酸封闭有害基= 因的表达。
=
=
=
= v 定义：
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•
=
=
=
Ø  4.  免疫基&#= 22240;治疗：将免疫增强因子基= 因= = (如= MHC，= IL)导入肿瘤细胞，提高= ;其免疫原= 性。
=
=
=
<= span style=3D'font-size:133%;color:#3366FF'>Ø= ;   5. 活化前体药物基因治疗： =
       向肿瘤细胞导入一种= ;基因，该基因产物为= 一种酶，它&= #21487;将无细胞毒的药物= 前体转化为有毒&#= 24615;的药物，从而将细胞杀死，= ;也称自杀基因= 治疗。 <= /span>
=
=
=
<= span style=3D'font-size:133%;color:#003BF8'>Ø= ;   6. 耐药基&#= 22240;治疗：
     将抗药物毒性的基= 因导入对化疗药物敏感= ;<= span style=3D'font-family:SimHei;mso-fareast-font-family:SimHei;font-size:133%'= >的组织细胞= (如骨髓干细胞= )，提高其对药物的= 耐受性。
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=
=
=
Ø1. 目的基因的表达水平太低或难&#= 20110;= 精确控制&#= 12290;
=
=
=
Ø2. 目的基因的转移和表达缺乏足够的 =
      靶向性。
=
=
=
Ø3. 对基因= 4920;达调控、基因表&#= 36798;产物= = 的功能的认识还&#= 24456;少、很肤浅。
目前&= #22522;因治疗面临的问题
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<= /div>
<= /div>
<= /div>
 &= #39029;眉
<= /div>
<= /div>
<= /div>
‹&= #26085;期/时间›
单击此处编辑母版ă= 91;本样式
第二级
第三级
第四级
第五级
<= /div>
<= /div>
<= /div>
‹<= /span>&= #39029;脚›
<= /div>
<= /div>
<= /div>
‹#›
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sld=3DGetObj("SlideObj") if( !g_supportsPPTHTML ) { =09 sld.style.visibility=3D"visible" return } if( MakeNotesVis() ) return runAnimations =3D _InitAnimations(); =09 if( IsWin("PPTSld") ) parent.SldUpdated(GetSldId()) g_origSz=3DparseInt(SlideObj.style.fontSize) g_origH=3Dsld.style.posHeight g_origW=3Dsld.style.posWidth g_scaleHyperlinks=3D(document.all.tags("AREA").length>0) if( g_scaleHyperlinks ) InitHLinkArray() if( g_scaleInFrame||(IsWin("PPTSld") && parent.IsFullScrMode() ) ) document.body.scroll=3D"no" _RSW() if( IsWin("PPTSld") && parent.IsFullScrMode() ) FullScrInit(); =09 MakeSldVis(); ChkAutoAdv() if( runAnimations ) { if( document.all("NSPlay") ) document.all("NSPlay").autoStart =3D false; if( sld.filters && sld.filters.revealtrans ) setTimeout( "document.body.start()", sld.filters.revealtrans.duration * = 1000 ); else document.body.start(); } } function MakeSldVis()=20 { var fTrans=3Dg_showAnimation && SldHasTrans() if( fTrans )=09 { if( g_bgSound ) { idx=3Dg_bgSound.indexOf(","); pptSound.src=3Dg_bgSound.substr( 0, idx ); pptSound.loop=3D -(parseInt(g_bgSound.substr(idx+1))); } SlideObj.filters.revealtrans.Apply()=09 } SlideObj.style.visibility=3D"visible" if( fTrans ) SlideObj.filters.revealtrans.Play() } function MakeNotesVis()=20 { if( !IsNts() ) return false=20 SlideObj.style.display=3D"none" nObj =3D document.all.item("NotesObj") parent.SetHasNts(0) if( nObj ) {=20 nObj.style.display=3D"" parent.SetHasNts(1) } return 1 } function ChkAutoAdv() { if(SldHasTrans()) SlideObj.onfilterchange=3DAutoAdv else AutoAdv() } function AutoAdv() { if(!IsWin("PPTSld") || !gUseSldTimings )return var sld=3DGetCurSld() if( (sld.mAdvDelay>0) && !parent.IsFramesMode() ) setTimeout("parent.GoToNextSld()",sld.mAdvDelay) } function GetObj(id) { if(g_supportsPPTHTML) return document.all(id); else return document.getElementById(id); } function SldHasTrans() { return SlideObj.style.filter !=3D ""; } function GetSldId() { return sId=3Dlocation.href.substring(location.href.la= stIndexOf('/')+1) } function HideMenu() { if( frames["PPTSld"] && PPTSld.document.all.item("ctx= tmenu") && PPTSld.ctxtmenu.style.display!=3D"none" ) { PPTSld.ctxtmenu.styl= e.display=3D'none'; return true } return false } function IsWin( name ) { return window.name =3D=3D name } function IsNts() { return IsWin("PPTNts") } function IsSldOrNts() { return( IsWin("PPTSld")||IsWin("PPTNts") ) } function SupportsPPTAnimation() { return( navigator.platform =3D=3D "Win32"= && navigator.appVersion.indexOf("Windows")>0 ) } function SupportsPPTHTML() { var appVer=3Dnavigator.appVersion, msie=3DappVer.indexOf("MSIE "), ver=3D0 if( msie >=3D 0 ) ver=3DparseFloat( appVer.substring( msie+5, appVer.indexOf(";",msie) ) ) else ver=3DparseInt(appVer) return( ver >=3D 4 && msie >=3D 0 ) } function _RSW() { if( !g_supportsPPTHTML || IsNts() || ( !g_scaleInFrame && (!IsWin("PPTSld") || !parent.IsFullScrMode()) ) ) return var padding=3D0; if( IsWin("PPTSld") && parent.IsFramesMode() ) padding=3D6 cltWidth=3Ddocument.body.clientWidth-padding cltHeight=3Ddocument.body.clientHeight-padding factor=3D(1.0*cltWidth)/g_origW if( cltHeight < g_origH*factor ) factor=3D(1.0*cltHeight)/g_origH newSize =3D g_origSz * factor if( newSize < 1 ) newSize=3D1 s=3DSlideObj.style s.fontSize=3DnewSize+"px" s.posWidth=3Dg_origW*factor s.posHeight=3Dg_origH*factor s.posLeft=3D(cltWidth-s.posWidth+padding)/2 s.posTop=3D(cltHeight-s.posHeight+padding)/2 if( g_scaleHyperlinks ) ScaleHyperlinks( factor ) } function _InitAnimations() { animRuntimeInstalled =3D ''+document.body.localTime !=3D 'undefined'; isFullScreen =3D (window.name =3D=3D "PPTSld") && !parent.IsFramesMode(); g_animUseRuntime =3D g_showAnimation && animRuntimeInstalled && !(isFullSc= reen && parent.IsSldVisited()); if( g_animUseRuntime ) { collSeq =3D document.all.tags("seq"); if( collSeq !=3D null ) { for(ii=3D0;ii numSlds ) gSldJumpIdx =3D numSlds; if ( gSldJumpIdx >=3D 0 ) { if ( gSldJumpIdx =3D=3D 0 ) gSldJumpIdx =3D 1; var jumpTo =3D parseInt(gSldJumpIdx); gSldJump =3D 0; gSldJumpIdx =3D ""; win.GoToSld( parent.GetSldList().mList[jumpTo-1].mSldHref ) } } } function _KDH() { if( event.keyCode =3D=3D 8 ) { event.returnValue =3D 0; parent.GoToPrevSld(); } }function DocumentOnClick() { if( IsNts() || parent.HideMenu() ) return; if( ( g_allowAdvOnClick && (window.name=3D=3D"PPTSld") && !parent.IsFrames= Mode() ) || (event && event.keyCode=3D=3D32) ) { =09 if( g_animUseRuntime && g_animMainSequence && g_animMainSequence.cangonex= t ) return; parent.GoToNextSld(); } } var g_supportsPPTHTML =3D SupportsPPTHTML(), g_scaleInFrame =3D 1, gId=3D""= , g_bgSound=3D"", g_scaleHyperlinks =3D false, g_allowAdvOnClick =3D 1, g_showInBrowser = =3D 0, gLoopCont =3D 0, gUseSldTimings =3D 1; var g_showAnimation =3D g_supportsPPTHTML && SupportsPPTAnimation() && ( (w= indow.name=3D=3D"PPTSld" && !parent.IsFramesMode()) || g_showInBrowser );va= r g_animManager =3D null; var g_animUseRuntime =3D false; var g_animItemsToHide, g_animInteractiveItems, g_animSlideTime; var g_animMainSequence =3D null; var ENDSHOW_MESG=3D"放映结束，单击= ;鼠标退出。", SCREEN_MODE=3D"Frames", gIsEndS= how=3D0, NUM_VIS_SLDS=3D72, SCRIPT_HREF=3D"script.js", FULLSCR_HREF=3D"full= screen.htm"; var gCurSld =3D gPrevSld =3D 1, g_offset =3D 0, gNtsOpen =3D gHasNts =3D gO= tlTxtExp =3D 0, gHasNarration =3D 0, gOtlOpen =3D true window.gPPTHTML=3DSupportsPPTHTML() var gMainDoc=3Dnew Array(new hrefList("slide0002.htm",1,-1,1),new hrefList(= "slide0003.htm",1,-1,1),new hrefList("slide0077.htm",1,-1,1),new hrefList("= slide0075.htm",1,-1,1),new hrefList("slide0073.htm",1,-1,1),new hrefList("s= lide0074.htm",1,-1,1),new hrefList("slide0076.htm",1,-1,1),new hrefList("sl= ide0004.htm",1,-1,1),new hrefList("slide0005.htm",1,-1,1),new hrefList("sli= de0006.htm",1,-1,1),new hrefList("slide0007.htm",1,-1,1),new hrefList("slid= e0008.htm",1,-1,1),new hrefList("slide0009.htm",1,-1,1),new hrefList("slide= 0010.htm",1,-1,1),new hrefList("slide0011.htm",1,-1,1),new hrefList("slide0= 012.htm",1,-1,1),new hrefList("slide0013.htm",1,-1,1),new hrefList("slide00= 14.htm",1,-1,1),new hrefList("slide0015.htm",1,-1,1),new hrefList("slide001= 6.htm",1,-1,1),new hrefList("slide0017.htm",1,-1,1),new hrefList("slide0018= .htm",1,-1,1),new hrefList("slide0019.htm",1,-1,1),new hrefList("slide0020.= htm",1,-1,1),new hrefList("slide0021.htm",1,-1,1),new hrefList("slide0022.h= tm",1,-1,1),new hrefList("slide0042.htm",1,-1,1),new hrefList("slide0043.ht= m",1,-1,1),new hrefList("slide0044.htm",1,-1,1),new hrefList("slide0045.htm= ",1,-1,1),new hrefList("slide0046.htm",1,-1,1),new hrefList("slide0047.htm"= ,1,-1,1),new hrefList("slide0048.htm",1,-1,1),new hrefList("slide0049.htm",= 1,-1,1),new hrefList("slide0050.htm",1,-1,1),new hrefList("slide0051.htm",1= ,-1,1),new hrefList("slide0052.htm",1,-1,1),new hrefList("slide0053.htm",1,= -1,1),new hrefList("slide0054.htm",1,-1,1),new hrefList("slide0055.htm",1,-= 1,1),new hrefList("slide0056.htm",1,-1,1),new hrefList("slide0057.htm",1,-1= ,1),new hrefList("slide0058.htm",1,-1,1),new hrefList("slide0059.htm",1,-1,= 1),new hrefList("slide0062.htm",1,-1,1),new hrefList("slide0063.htm",1,-1,1= ),new hrefList("slide0060.htm",1,-1,1),new hrefList("slide0061.htm",1,-1,1)= ,new hrefList("slide0064.htm",1,-1,1),new hrefList("slide0065.htm",1,-1,1),= new hrefList("slide0066.htm",1,-1,1),new hrefList("slide0067.htm",1,-1,1),n= ew hrefList("slide0069.htm",1,-1,1),new hrefList("slide0070.htm",1,-1,1),ne= w hrefList("slide0023.htm",1,-1,1),new hrefList("slide0024.htm",1,-1,1),new= hrefList("slide0025.htm",1,-1,1),new hrefList("slide0026.htm",1,-1,1),new = hrefList("slide0028.htm",1,-1,1),new hrefList("slide0029.htm",1,-1,1),new h= refList("slide0030.htm",1,-1,1),new hrefList("slide0031.htm",1,-1,1),new hr= efList("slide0033.htm",1,-1,1),new hrefList("slide0034.htm",1,-1,1),new hre= fList("slide0035.htm",1,-1,1),new hrefList("slide0036.htm",1,-1,1),new href= List("slide0037.htm",1,-1,1),new hrefList("slide0072.htm",1,-1,1),new hrefL= ist("slide0038.htm",1,-1,1),new hrefList("slide0039.htm",1,-1,1),new hrefLi= st("slide0040.htm",1,-1,1),new hrefList("slide0041.htm",1,-1,1)); /********************************************* Frameset functions These functions control slide navigation and state of the frameset. **********************************************/ function FullScrInit() { g_allowAdvOnClick =3D GetCurSld().mAdvOnClk document.body.style.backgroundColor=3D"black" document.oncontextmenu=3Dparent._CM; document.onkeydown =3D _KDH; document.ondragstart=3DCancel document.onselectstart=3DCancel self.focus() } function Redirect( frmId ) {=09 var str=3Ddocument.location.hash,idx=3Dstr.indexOf('#'), sId=3DGetSldId() if(idx>=3D0) str=3Dstr.substr(1); if( window.name !=3D frmId && ( sId !=3D str) ) { obj =3D GetObj("Main-File") window.location.href=3Dobj.href+"#"+sId return 1 } return 0 } var MHTMLPrefix =3D CalculateMHTMLPrefix();=20 function CalculateMHTMLPrefix() { if ( document.location.protocol =3D=3D 'mhtml:') {=20 href=3Dnew String(document.location.href)=20 Start=3Dhref.indexOf('!')+1=20 End=3Dhref.lastIndexOf('/')+1=20 if (End < Start)=20 return href.substring(0, Start)=20 else=20 return href.substring(0, End)=20 } return ''; } function GetTags(base,tag) { if(g_supportsPPTHTML) return base.all.tags(tag); else return base.getElementsByTagName(tag); } function UpdNtsPane(){ if(frames["PPTNts"]) PPTNts.location.replace( MHTMLP= refix+GetHrefObj( gCurSld ).mNtsHref ) } function UpdNavPane( sldIndex ){ if(gNavLoaded) PPTNav.UpdNav() } function UpdOtNavPane(){ if(gOtlNavLoaded) PPTOtlNav.UpdOtlNav() } function UpdOtlPane(){ if(gOtlLoaded) PPTOtl.UpdOtl() } function SetHasNts( fVal ) { if( gHasNts !=3D fVal ) { gHasNts=3DfVal UpdNavPane() } } function ToggleOtlText() { gOtlTxtExp=3D!gOtlTxtExp UpdOtlPane() } function ClearMedia() { // Clear any sounds playing before launching another browser window. Other= wise, // in fullscreen mode, you'll continue to hear the sound in the frames mod= e. if (PPTSld.pptSound) PPTSld.pptSound.loop =3D 0; } function FullScreen() {=20 if ( PPTSld.g_animUseRuntime ) PPTSld.document.body.pause(); ClearMedia(); var href =3D ( document.location.protocol =3D=3D 'mhtml:') ? FULLSCR_HREF = : FULLSCR_HREF+"#"+GetHrefObj(gCurSld).mSldHref; if(PPTNav.event.ctrlKey) { var w =3D (window.screen.availWidth * 1.0) / 2.0 var h =3D w * (PPTSld.g_origH * 1.0) / PPTSld.g_origW win =3D window.open( MHTMLPrefix+href,null,"toolbar=3D0,resizable=3D1,top= =3D0,left=3D0," + "width=3D"+ w + ",height=3D" + h ); if( win.document.body && PPTSld.g_animUseRuntime ) win.document.body.PPTSldFrameset=3Dwindow; } else { win =3D window.open( MHTMLPrefix+href,null,"fullscreen=3Dyes" ); if( win.document.body && PPTSld.g_animUseRuntime ) win.document.body.PPTSldFrameset=3Dwindow; } } function ToggleVNarration() { rObj=3DPPTSld.document.all("NSPlay") if( rObj && !PPTSld.g_animUseRuntime ) { if( (rObj.playState =3D=3D 1)||(rObj.playState =3D=3D 0) ) rObj.Play() else if( rObj.playState =3D=3D 2 ) rObj.Pause() else return; } else if( PPTSld.g_animUseRuntime ) { narObj =3D PPTSld.document.all("narrationID") if( narObj ) narObj.togglePause() } } function GetCurSldNum() { =20 obj=3DGetHrefObj(gCurSld) if( obj.mOrigVis =3D=3D 1 ) return obj.mSldIdx else =20 return gCurSld } function GetNumSlds() { =20 if( GetHrefObj(gCurSld).mOrigVis =3D=3D 1 ) return GetSldList().mNumVisSlds; else return GetSldList().mList.length } function GetSldNum( href ) { for(ii=3D0; ii 1 ) PopSldList(); else if( !IsFramesMode() ) { if( gLoopCont ) GoToFirst() else EndShow() } } function GoToPrevSld() { ii=3DgCurSld-1 if( ii > 0 ) { obj=3DGetHrefObj(ii) while ( obj && ( obj.mVis =3D=3D 0 ) && ( ii>0 ) ) obj=3DGetHrefObj(--ii) if( ii =3D=3D 0 ) ii=3D1 GoToSldNum(ii) } } function GoToFirst(){ GoToSld( GetHrefObj(1).mSldHref ) } function GoToLast() { ii=3DGetSldList().mList.length if( ii !=3D gCurSld ) GoToSld( GetHrefObj(ii).mSldHref ) } function GoToSldNum( num ) { if( PPTSld.event ) PPTSld.event.cancelBubble=3Dtrue obj =3D GetHrefObj( num ) obj.mVis=3D1 gPrevSld=3DgCurSld gCurSld =3D num; PPTSld.location.replace(MHTMLPrefix+obj.mSldHref) if( IsFramesMode() ) { UpdNavPane(); UpdOtlPane(); UpdNtsPane() } } function GoToSld( href ) { if( PPTSld.event ) PPTSld.event.cancelBubble=3Dtrue GetHrefObj( GetSldNum(href) ).mVis=3D1 PPTSld.location.replace(MHTMLPrefix+href) } function SldUpdated( id ) { if( id =3D=3D GetHrefObj(gCurSld).mSldHref ) return gPrevSld=3DgCurSld gCurSld=3DGetSldNum(id) if( IsFramesMode() ) { UpdNavPane(); UpdOtlPane(); UpdNtsPane() } } function PrevSldViewed(){ GoToSld( GetHrefObj(gPrevSld).mSldHref ) } function HasPrevSld() { return ( gIsEndShow || ( gCurSld !=3D 1 && GetHrefO= bj( gCurSld-1 ).mVis =3D=3D 1 )||( GetCurSldNum() > 1 ) ) } function HasNextSld() { return (GetCurSldNum() !=3D GetNumSlds()) } function CloseWindow() { if( HideMenu() ) return; =09 var event =3D PPTSld.event; if( !IsFramesMode() && event && (event.keyCode=3D=3D27 || event.keyCode=3D= =3D32 || event.type=3D=3D"click" ) ) window.close( self ); CatchNumKeys( self, event ); } function Unload() { gIsEndShow=3D0; } function SetupEndShow() { gIsEndShow=3D1; PPTSld.document.body.scroll=3D"no"; PPTSld.document.onkeypress=3DCloseWindow; PPTSld.document.onclick=3DCloseWindow; PPTSld.document.oncontextmenu=3D_CM; } function EndShow() { if( IsFramesMode() ) return if( PPTSld.event ) PPTSld.event.cancelBubble=3Dtrue doc=3DPPTSld.document var dir =3D doc.body.dir if( dir !=3D "rtl" ) dir =3D "ltr"; doc.open() doc.writeln('


' + ENDSHOW_MESG + '

') doc.close() } function SetSldVisited(){ GetSldList().mList[gCurSld-1].mVisited=3Dtrue } function IsSldVisited(){ return GetSldList().mList[gCurSld-1].mVisited } function hrefList( sldHref, visible, advDelay, advClk ) { this.mSldHref=3D this.mNtsHref =3D sldHref this.mOrigVis=3D this.mVis =3D visible this.mVisited=3D false this.mAdvDelay=3D advDelay this.mAdvOnClk=3D advClk } function SldList(arr,curSld,fEnd) { this.mCurSld =3D curSld; this.mList =3D new Array(); var idx =3D 1; for(ii=3D0;ii 0) { PushSldList(sldList,fEnd); gCurSld =3D 1; } else if( PPTSld.event ) PPTSld.event.cancelBubble=3Dtrue } function PushSldList(arr,fEnd) { var ii =3D gSldStack.length; gSldStack[ii] =3D new SldList(arr,gCurSld,fEnd); GoToSld( gSldStack[ii].mList[0].mSldHref ); } function PopSldList() { if (gSldStack[gSldStack.length-1].fEndShow) EndShow() else { gCurSld =3D gSldStack[gSldStack.length-1].mCurSld; gSldStack[gSldStack.length-1] =3D null; gSldStack.length--; var sldList =3D gSldStack[gSldStack.length-1]; GoToSld( sldList.mList[gCurSld - 1].mSldHref ); } } var custShowList=3Dnew Array(); /********************************************* Navigation button implementation There are 2 types of buttons: ImgBtn, TxtBtn implemented as function objects. They share a similiar interface so the event handlers can call SetActive, for example, on a button=20 object without needing to know exactly=20 what type of button it is. **********************************************/ //---------------------------------- function ImgBtn( oId,bId,w,action ) //---------------------------------- { var t=3Dthis t.Perform =3D _IBP t.SetActive =3D _IBSetA t.SetInactive=3D _IBSetI t.SetPressed =3D _IBSetP t.SetDisabled=3D _IBSetD t.Enabled =3D _IBSetE t.ChangeIcon =3D null t.UserAction =3D action t.ChgState =3D _IBUI t.mObjId =3D oId t.mBorderId=3D bId t.mWidth =3D w t.mIsOn =3D t.mCurState =3D 0 } function _IBSetA() { if( this.mIsOn ) { obj=3Dthis.ChgState( gHiliteClr,gShadowClr,2 ) obj.style.posTop=3D0 } } function _IBSetI() { if( this.mIsOn ) { obj=3Dthis.ChgState( gFaceClr,gFaceClr,1 ) obj.style.posTop=3D0=20 } } function _IBSetP() { if( this.mIsOn ) { obj=3Dthis.ChgState( gShadowClr,gHiliteClr,2 ) obj.style.posLeft+=3D1; obj.style.posTop+=3D1 } } function _IBSetD() { =20 obj=3Dthis.ChgState( gFaceClr,gFaceClr,0 ) obj.style.posTop=3D0=20 } function _IBSetE( state ) { var t=3Dthis GetObj( t.mBorderId ).style.visibility=3D"visible" if( state !=3D t.mIsOn ) { t.mIsOn=3Dstate if( state ) t.SetInactive() else t.SetDisabled() } } function _IBP() { var t=3Dthis if( t.mIsOn ) { if( t.UserAction !=3D null ) t.UserAction() if( t.ChangeIcon ) { obj=3DGetObj(t.mObjId) if( t.ChangeIcon() ) obj.style.posLeft=3Dobj.style.posLeft+(t.mCurState-4)*t.mWidth else obj.style.posLeft=3Dobj.style.posLeft+(t.mCurState-0)*t.mWidth } t.SetActive() } =20 } function _IBUI( clr1,clr2,nextState ) { var t=3Dthis SetBorder( GetObj( t.mBorderId ),clr1,clr2 ) obj=3DGetObj( t.mObjId ) obj.style.posLeft=3Dobj.style.posLeft+(t.mCurState-nextState)*t.mWidth-obj= .style.posTop t.mCurState=3DnextState return obj } //----------------------------------------- function TxtBtn( oId,oeId,action,chkState ) //----------------------------------------- { var t=3Dthis t.Perform =3D _TBP t.SetActive =3D _TBSetA t.SetInactive=3D _TBSetI t.SetPressed =3D _TBSetP t.SetDisabled=3D _TBSetD t.SetEnabled =3D _TBSetE t.GetState =3D chkState t.UserAction =3D action t.ChgState =3D _TBUI t.mObjId =3D oId t.m_elementsId=3D oeId t.mIsOn =3D 1 } function _TBSetA() { var t=3Dthis if( t.mIsOn && !t.GetState() ) t.ChgState( gHiliteClr,gShadowClr,0,0 ) } function _TBSetI() { var t=3Dthis if( t.mIsOn && !t.GetState() ) t.ChgState( gFaceClr,gFaceClr,0,0 ) } function _TBSetP() { if( this.mIsOn ) this.ChgState( gShadowClr,gHiliteClr,1,1 ) } function _TBSetD() { =20 this.ChgState( gFaceClr,gFaceClr,0,0 ) this.mIsOn =3D 0 } function _TBSetE() { var t=3Dthis if( !t.GetState() ) t.ChgState( gFaceClr,gFaceClr,0,0 ) else t.ChgState( gShadowClr,gHiliteClr,1,1 ) t.mIsOn =3D 1 } function _TBP() { var t=3Dthis if( t.mIsOn ) {=20 if( t.UserAction !=3D null ) t.UserAction() if( !t.GetState ) return if( t.GetState() ) t.SetPressed() else t.SetActive() } =20 } function _TBUI( clr1,clr2,lOffset,tOffset ) { SetBorder( GetObj( this.mObjId ),clr1,clr2 ) Offset( GetObj( this.m_elementsId ),lOffset,tOffset ) } function Offset( obj, top, left ){ obj.style.top=3Dtop; obj.style.left=3Dle= ft } function SetBorder( obj, upperLeft, lowerRight ) { s=3Dobj.style; s.borderStyle =3D "solid" s.borderWidth =3D 1=20 s.borderLeftColor =3D s.borderTopColor =3D upperLeft s.borderBottomColor=3D s.borderRightColor =3D lowerRight } function GetBtnObj(){ return gBtnArr[window.event.srcElement.id] } function BtnOnOver(){ b=3DGetBtnObj(); if( b !=3D null ) b.SetActive() } function BtnOnDown(){ b=3DGetBtnObj(); if( b !=3D null ) b.SetPressed() } function BtnOnOut(){ b=3DGetBtnObj(); if( b !=3D null ) b.SetInactive() } function BtnOnUp() { b=3DGetBtnObj() if( b !=3D null ) b.Perform() else Upd() } function GetNtsState(){ return parent.gNtsOpen } function GetOtlState(){ return parent.gOtlOpen } function GetOtlTxtState(){ return parent.gOtlTxtExp } function NtsBtnSetFlag( fVal ) { s=3Ddocument.all.item( this.m_flagId ).style s.display=3D"none" if( fVal ) s.display=3D"" else s.display=3D"none" } function _BSetA_Border(){ b =3D gBtnArr[this.mObjId]; if( b !=3D null ) b.S= etActive() } function _BSetI_Border(){ b =3D gBtnArr[this.mObjId]; if( b !=3D null ) b.S= etInactive() } function _BSetP_Border(){ b =3D gBtnArr[this.mObjId]; if( b !=3D null ) b.S= etPressed() } function _BSetA_BorderImg() {=20 b =3D gBtnArr[this.mBorderId]=20 if( b !=3D null && this.mIsOn && !b.GetState() ) { obj=3Dthis.ChgState( gHiliteClr,gShadowClr,2 ) obj.style.posTop=3D0 } } function _BSetI_BorderImg() {=20 b =3D gBtnArr[this.mBorderId] if( b !=3D null && this.mIsOn && !b.GetState() ) { obj=3Dthis.ChgState( gFaceClr,gFaceClr,1 ) obj.style.posTop=3D0 } } var gHiliteClr=3D"THREEDHIGHLIGHT",gShadowClr=3D"THREEDSHADOW",gFaceClr=3D"= THREEDFACE" var gBtnArr =3D new Array() gBtnArr["nb_otl"] =3D new TxtBtn( "nb_otl","nb_otlElem",parent.ToggleOtlPan= e,GetOtlState ) gBtnArr["nb_otlElem"] =3D new TxtBtn( "nb_otl","nb_otlElem",parent.ToggleOt= lPane,GetOtlState ) gBtnArr["nb_nts"] =3D new ImgBtn( "nb_nts","nb_ntsBorder",10,parent.ToggleN= tsPane ) gBtnArr["nb_nts"].SetActive =3D _BSetA_BorderImg; gBtnArr["nb_nts"].SetInactive =3D _BSetI_BorderImg; gBtnArr["nb_ntsBorder"] =3D new TxtBtn( "nb_ntsBorder","nb_ntsElem",parent.= ToggleNtsPane,GetNtsState ) gBtnArr["nb_ntsElem"] =3D new TxtBtn( "nb_ntsBorder","nb_ntsElem",parent.To= ggleNtsPane,GetNtsState ) gBtnArr["nb_prevBorder"] =3D gBtnArr["nb_prev"]=3D new ImgBtn( "nb_prev","n= b_prevBorder",30,parent.GoToPrevSld ) gBtnArr["nb_nextBorder"] =3D gBtnArr["nb_next"]=3D new ImgBtn( "nb_next","n= b_nextBorder",30,parent.GoToNextSld ) gBtnArr["nb_sldshw"]=3D new ImgBtn( "nb_sldshw","nb_sldshwBorder",18,parent= .FullScreen ) gBtnArr["nb_sldshwBorder"] =3D new TxtBtn( "nb_sldshw","nb_sldshwBorder",pa= rent.FullScreen,null ) gBtnArr["nb_sldshwBorder"].SetActive =3D _BSetA_Border; gBtnArr["nb_sldshwBorder"].SetInactive =3D _BSetI_Border; gBtnArr["nb_sldshwText"] =3D new TxtBtn( "nb_sldshw","nb_sldshwText",parent= .FullScreen,null ) gBtnArr["nb_sldshwText"].SetActive =3D _BSetA_Border; gBtnArr["nb_sldshwText"].SetInactive =3D _BSetI_Border; gBtnArr["nb_voice"] =3D gBtnArr["nb_voiceBorder"] =3D new ImgBtn( "nb_voice= ","nb_voiceBorder",18,parent.ToggleVNarration ) gBtnArr["nb_otlTxtBorder"] =3D gBtnArr["nb_otlTxt"]=3D new ImgBtn( "nb_otlT= xt","nb_otlTxtBorder",23,parent.ToggleOtlText ) gBtnArr["nb_ntsBorder"].m_flagId=3D "nb_nts" gBtnArr["nb_ntsBorder"].SetFlag =3D NtsBtnSetFlag gBtnArr["nb_otlTxt"].ChangeIcon=3D GetOtlTxtState /********************************************* Context menu implementation _CM() is the function that's hooked up to the oncontextmenu event. Once we're asked to show the menu, we first build it by creating DIVs on-the-fly. Then we position it=20 within the screen area so it doesn't get clipped. Creating the DIVs using createElement() means we don't have to write out any extra HTML into the slide HTML files. **********************************************/ var sNext=3D"下一步",sPrev=3D"上一步",s= End=3D"结束放映",sFont=3D"宋体",sArrow= =3D"箭头",sFreeform=3D"任意形状",sRect= =3D"矩形",sOval=3D"椭圆" function ShowMenu() { BuildMenu(); var doc=3DPPTSld.document.body,x=3DPPTSld.event.clientX+doc.scrollLeft,y= =3DPPTSld.event.clientY+doc.scrollTop m =3D PPTSld.document.all.item("ctxtmenu") m.style.pixelLeft=3Dx if( (x+m.scrollWidth > doc.clientWidth)&&(x-m.scrollWidth > 0) ) m.style.pixelLeft=3Dx-m.scrollWidth m.style.pixelTop=3Dy if( (y+m.scrollHeight > doc.clientHeight)&&(y-m.scrollHeight > 0) ) m.style.pixelTop=3Dy-m.scrollHeight m.style.display=3D"" } function _CM() { if( !parent.IsFullScrMode() ) return; if(!PPTSld.event.ctrlKey) { ShowMenu() return false } else HideMenu() } function BuildMenu() { if( PPTSld.document.all.item("ctxtmenu") ) return var mObj=3DCreateItem( PPTSld.document.body ) mObj.id=3D"ctxtmenu" mObj.style.visibility=3D"hidden" var s=3DmObj.style s.position=3D"absolute" s.cursor=3D"default" s.width=3D"120px" SetCMBorder(mObj,"menu","black") var iObj=3DCreateItem( mObj ) SetCMBorder( iObj, "threedhighlight","threedshadow" ) iObj.style.padding=3D2 CreateMenuItem( iObj,sNext,M_GoNextSld,M_True ) CreateMenuItem( iObj,sPrev,M_GoPrevSld,M_HasPrevSld ) =09 CreateSeparator( iObj ) CreateMenuItem( iObj,sEnd,M_End,M_True ) mObj.style.visibility=3D"visible" } function Cancel() { window.event.cancelBubble=3Dtrue; window.event.returnVa= lue=3Dfalse } function Highlight() { ChangeClr("activecaption","threedhighlight") } function Deselect() { ChangeClr("threedface","menutext") } function Perform() { e=3DPPTSld.event.srcElement if( e.type=3D=3D"menuitem" && e.IsActive() ) e.Action() else PPTSld.event.cancelBubble=3Dtrue } function ChangeClr( bg,clr ) { e=3DPPTSld.event.srcElement if( e.type=3D=3D"menuitem" && e.IsActive() ) { e.style.backgroundColor=3Dbg e.style.color=3Dclr } } function M_HasPrevSld() { return( parent.HasPrevSld() ) } function M_GoNextSld() { if( gIsEndShow ) M_End(); else GoToNextSld() } function M_GoPrevSld() { if( gIsEndShow ) { gIsEndShow=3D0; history.back();= PPTSld.event.cancelBubble=3Dtrue; } else GoToPrevSld() } function M_True() { return true } function M_End() { window.close( self ) } function CreateMenuItem( node,text,action,eval ) { var e=3DCreateItem( node ) e.type=3D"menuitem" e.Action=3Daction e.IsActive=3Deval e.innerHTML=3Dtext if( !e.IsActive() ) e.style.color=3D"threedshadow" e.onclick=3DPerform e.onmouseover=3DHighlight e.onmouseout=3DDeselect s=3De.style; s.fontFamily=3DsFont s.fontSize=3D"9pt" s.paddingLeft=3D2 } function CreateSeparator( node ) { var sObj=3DCreateItem( node ) SetCMBorder(sObj,"menu","menu") var s=3DsObj.style s.borderTopColor=3D"threedshadow" s.borderBottomColor=3D"threedhighlight" s.height=3D1 s.fontSize=3D"0px" } function CreateItem( node ) { var elem=3DPPTSld.document.createElement("DIV") node.insertBefore( elem ) return elem } function SetCMBorder( o,ltClr,rbClr ) { var s=3Do.style s.backgroundColor=3D"menu" s.borderStyle=3D"solid" s.borderWidth=3D1 s.borderColor=3DltClr+" "+rbClr+" "+rbClr+" "+ltClr } ------=_NextPart_01C48431.FDF062B0 Content-Location: file:///C:/85899A55/15.files/fullscreen.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii"
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